灵芝多糖的分离纯化、结构表征和抗氧化活性研究  

Isolation,purification,structural characterization and antioxidant activity of Ganoderma lucidum polysaccharides

作　　者：刘丹[1] 任洪飞 逄梦玉 隋昕怡 杜秀菊[1] LIU Dan;REN Hong-fei;PANG Meng-yu;SUI Xin-yi;DU Xiu-ju(College of Agriculture and Biology,Liaocheng University,Liaocheng 252000,Shandong,China)

机构地区：[1]聊城大学农业与生物学院,山东聊城252000

出　　处：《粮食与油脂》2025年第3期81-86,101,共7页Cereals & Oils

基　　金：国家自然科学基金(32300031);山东省自然科学基金项目(ZR2023MB095);聊城大学科研基金项目(318011707);大学生创新创业训练计划项目(202310447003S)。

摘　　要：采用反复冻融法制备灵芝多糖(GLPf),经乙醇分级得到GLPf30、GLPf60和GLPf803种多糖。选用活性最高的GLPf30为研究对象,采用CaptoTM DEAE离子柱层析获得GLPf303,再经Superdex 6 Prep Grad凝胶层析进行分离纯化,获得高纯度多糖GLPf303a。利用扫描电子显微镜(SEM)、傅里叶红外光谱、高效液相凝胶色谱等对其进行结构表征。结果表明:GLPf303a是以吡喃糖环为基本骨架的多糖,其重均分子量为18.72 kDa,由岩藻糖、鼠李糖、阿拉伯糖、氨基葡萄糖、半乳糖、葡萄糖、木糖、甘露糖和葡萄糖醛酸9种单糖组成。抗氧化试验结果表明,GLPf303a和GLPf303均对ABTS自由基具有较强的清除作用,且GLPf303a的羟基自由基清除率以及还原力均高于GLPf303。Ganoderma lucidum polysaccharides(GLPf)was prepared by repeated freeze-thaw method,GLPf30,GLPf60 and GLPf80 polysaccharides were obtained by ethanol grading.GLPf30 with the highest activity was selected as the research object.GLPf303 was obtained by CaptoTM DEAE ion column chromatography,and purified by Superdex 6 Prep Grad gel chromatography to obtain,high purity polysaccharide GLPf303a.The structures were characterized by scanning electron microscope(SEM),Fourier transform infrared spectrometer,and high performance liquid gel chromatography.The results showed that GLPf303a was a polysaccharide with a pyranose ring as the basic skeleton,and its weight average molar mass was 18.72 kDa.It was composed of 9 monosaccharides as fucose,rhamnoose,arabinose,glucosamine,galactose,glucose,xylose,mannose,and glucuronic acid.The results of antioxidant tests showed that both GLPf303a and GLPf303 had strong scavenging effects on ABTS free radicals,and the hydroxyl radical scavenging and reducing power of GLPf303a were higher than that of GLPf303.

关 键 词：灵芝多糖 阴离子层析 凝胶层析 结构表征 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]