菠萝皮渣发酵液光发酵制氢工艺参数优化  

Parameter optimization of photobiological hydrogen production process using fermented broth of pineapple peel residue

作　　者：屈皓 刘玉香[1] 李玉霞 QU Hao;LIU Yuxiang;LI Yuxia(College of Environmental Science and Engineering,Taiyuan University of Technology,Taiyuan 030001,China;Department of Chemistry and Chemical Engineering,Jinzhong University,Jinzhong 030619,China)

机构地区：[1]太原理工大学环境科学与工程学院,太原030001 [2]晋中学院化学化工系,山西晋中030619

出　　处：《农业环境科学学报》2025年第2期491-498,共8页Journal of Agro-Environment Science

基　　金：山西省基础研究计划项目(202103021224106)。

摘　　要：为探究混合光发酵菌群XW在菠萝皮渣发酵液中的产氢规律并优化其产氢条件,以累积产氢量为考察指标,通过Box-Behnken Design(BBD)响应面法对其主要影响因素的交互关系进行分析,并利用模型预测其最佳产氢条件。实验结果表明:初始pH和温度、初始pH和光发酵菌群接种量的交互作用对产氢量的影响显著,而温度与光发酵菌群接种量的交互作用对产氢量的影响相对较小;最佳工艺条件为初始pH6.93,光发酵菌群接种量22.86%,温度32.12℃,模型预测最大产氢量为124.27mL,实际实验产氢量为126.13mL,相对误差为1.49%,说明该数值模型具有良好的拟合性。研究表明,菠萝皮渣发酵液是混合光发酵菌群进行制氢的优良底物,不同的初始pH、温度和菌群接种量对产氢效果均有影响,模型优化的最佳工艺条件具有良好的参考价值。This study aims to explore the hydrogen production law of the mixed photofermentative bacteria strain XW in a fermented broth made from pineapple peel residue and to optimize the hydrogen production conditions.Cumulative hydrogen production was used as the indicator to examine the interaction between major influencing factors using the Box-Behnken Design(BBD)response surface methodology.The optimal hydrogen production conditions were predicted using the model.The interactions between initial pH and temperature and the initial pH and inoculation amount of photofermentative bacteria had significant effects on hydrogen production,while the interaction between temperature and the inoculation amount of photofermentative bacteria had a relatively small impact on hydrogen production.The optimal process conditions were an initial pH of 6.93,an inoculation amount of 22.86%photofermentative bacteria,and a temperature of 32.12℃.The model predicted a maximum hydrogen production of 124.27 mL,while the actual experimental hydrogen production was 126.13 mL,with a relative error of 1.49%.This indicated that the numerical model had good fitting properties.Pineapple peel residue fermentation broth is an excellent substrate for hydrogen production by mixed photofermentative bacteria.Different initial pH,temperature,and inoculation amounts of bacteria can affect hydrogen production,and the optimal process conditions,as determined by optimized models,can be used as reference values.

关 键 词：光发酵制氢 工艺优化 响应面法 菠萝皮渣 发酵液 

分 类 号：TQ116.2[化学工程—无机化工] TS209[轻工技术与工程—食品科学] TQ920.6[轻工技术与工程—食品科学与工程]