基于HS-SPME-GC-MS法检测复合香型白酒酿造过程不同样品中乙偶姻的含量及其差异分析  

Determination of Acetoin Content and Difference Analysis in Different Samples of Compound Flavor Baijiu Brewing Process Based on HS-SPME-GC-MS

作　　者：李娜 程伟[1] 薛锡佳[1] 潘天全 代森 LI Na;CHENG Wei;XUE Xijia;PAN Tianquan;DAI Sen(Anhui Golden Seed Wine Industry Co.,Ltd.,Fuyang 236023,Anhui,China)

机构地区：[1]安徽金种子酒业股份有限公司,安徽阜阳236023

出　　处：《酿酒》2025年第2期73-76,共4页Liquor Making

基　　金：2023年度安徽省重点研究与开发计划项目(No.2023n06020032);2023年度企业博士后科研工作站计划项目(No.2023JKY-B01);2023年度企业产学研合作计划项目(No.2023JKY-HC1580)。

摘　　要：乙偶姻作为四甲基吡嗪的前体物质,其具有令人愉快的奶油香味,并对白酒风味具有重要影响。为探究复合香型白酒不同酿造工艺段样品中乙偶姻的含量分布及其差异,为发酵过程中四甲基吡嗪的溯源提供数据基础,本研究采用顶空固相微萃取结合气相色谱-质谱联用法(HS-SPME-GC-MS)和外标法对复合香型白酒酿造用大曲、麸曲、粮醅和酒醅进行乙偶姻的含量测定,并考察了检测方法的精密度和加标回收率。结果表明,检测方法的精密度和加标回收率均满足分析要求,中温大曲和麸曲中乙偶姻的含量高于高温大曲,堆积后粮醅中乙偶姻的含量有所增加,下层酒醅的乙偶姻含量明显高于上层酒醅。本研究有利于明确复合香型白酒不同酿造工艺段样品中乙偶姻的含量分布特点,对发酵过程中四甲基吡嗪的溯源具有重要意义。As the precursor of tetramethylpyrazine,acetoin has a pleasant cream flavor and has an important impact on the flavor of Baijiu.In order to explore the content distribution and difference of acetoin in the samples of different brewing process sections of Fuhexiang Baijiu and provide data basis for the traceability of tetramethylpyrazine during fermentation,this study used headspace solid phase microextraction combined with gas chromatography-mass spectrometry(HS-SPME-GC-MS)and external standard methods to determine the content of acetoin in Daqu,Fuqu,Liangpei and Jiupei for the brewing of Fuhexiang Baijiu,and investigated the precision and spiked recovery of the detection methods.The results showed that the precision and recovery rate of the detection method met the analysis requirements.The content of ethyl acetate in medium temperature Daqu and Fuqu was higher than that in high temperature Daqu.After stacking,the content of ethyl acetate in the grain mash increased,and the content of ethyl acetate in the lower layer of the mash was significantly higher than that in the upper layer of the mash.This study is conducive to clarifying the content distribution characteristics of acetoin in the samples of different brewing processes of Fuhexiang Baijiu,and has important significance for the traceability of tetramethylpyrazine in the fermentation process.

关 键 词：复合香型白酒 酿造过程 乙偶姻的含量 检测 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS207.3[轻工技术与工程—食品科学与工程] TS201.2