碘-淀粉显色法筛选α-淀粉酶抑制剂的方法研究  

Study of a Screening Method for α-amylase Inhibitors Using Iodine Starch Colorimetry

作　　者：杨敏 向灿辉 王文君 YANG Min;XIANG Canhui;WANG Wenjun(College of Bioengineering,Zhuhai Campus of Zunyi Medical University,Zhuhai 519041,China)

机构地区：[1]遵义医科大学珠海校区生物工程学院,广东珠海519041

出　　处：《山东化工》2025年第3期36-39,共4页Shandong Chemical Industry

基　　金：遵义市科技计划项目(遵市科合HZ字〔2020〕37号);遵义医学院学术新苗培养及创新探索专项(黔科合平台人才[2018]5772-044);黔科合基础〔2019〕1354号。

摘　　要：采用碘-淀粉显色法建立了α-淀粉酶抑制剂的筛选方法。对淀粉浓度、α-淀粉酶浓度、有机溶剂甲醇的用量和反应终止液盐酸浓度进行了逐一考察,结果表明最佳测定条件淀粉基质液质量浓度为0.0035 g·mL^(-1),淀粉酶基质液质量浓度为0.05 mg·mL^(-1),有机溶剂甲醇的体积分数不超过10%为宜,反应终止液盐酸浓度选用1.0 mol·L^(-1)最佳。以阿卡波糖为阳性对照,比较了对香豆酸、儿茶素和岩白菜素对α-淀粉酶的抑制活性,同浓度时对香豆酸对α-淀粉酶的抑制活性最强,并测得对香豆酸的IC_(50)值为2.79 mg·mL^(-1)。A screening method for α-amylase inhibitors was established based on the iodine-starch coloritmetry.Starch concentration,α-amylase concentration,the concentrations of organic solvent methanol and hydrochloric acid for stopping reaction were investigated one by one.The optimal determination conditions were described as follows:the concentration of starch matrix solution was 0.0035 g·mL^(-1),the concentration of amylase matrix solution was 0.05 mg·mL^(-1),the concentration of organic solvent methanol was not more than 10%,and the concentration of hydrochloric acid for terminated action was 1.0 mol·L^(-1).The inhibitory activity of p-coumaric acid,catechin and bergenin on α-amylase were investigated with the acarbose as the positive control drug,the results showed that the inhibitory activity of p-coumaric acid onα-amylase was the highest one,and the IC_(50) value of p-coumaric acid was 2.79 mg·mL^(-1).

关 键 词：碘-淀粉显色法 Α-淀粉酶抑制剂 对香豆素 儿茶素 岩白菜素 

分 类 号：TQ925[轻工技术与工程—发酵工程] TS201.2