基于三维肠道细胞模型的宿主与食源性致病菌互作的研究进展  

Research progress on host-foodborne pathogen interactions based on three-dimensional intestinal cell models

作　　者：徐晓庆 夏雪娟 宋仪洋 董庆利[1] 李卓思 XU Xiaoqing;XIA Xuejuan;SONG Yiyang;DONG Qingli;LI Zhuosi(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093

出　　处：《食品与发酵工业》2025年第6期378-389,共12页Food and Fermentation Industries

基　　金：上海市浦江人才计划项目(23PJ1409600);国家自然科学基金项目(32200078);国家重点研发计划政府间国际科技创新合作重点专项(2024YFE0102600)。

摘　　要：体外肠道模型是研究食源性致病菌与宿主相互作用的重要工具。传统二维肠道细胞模型是最常用的体外培养模型,但是由于其不能有效模拟人体的生理特征,存在一定的局限性。新型三维肠道细胞模型,能更好地模拟人体肠道的微结构、生理功能和微环境,已逐渐成为研究肠道生理病理、肠道微生物群、病原体与宿主互作的有力工具。该文首先介绍肠道的基本构造和主要细胞亚型的特征;其次,分析并讨论了典型食源性致病菌与宿主之间的相互作用;最后,重点综述并讨论了多细胞球体、肠道类器官、器官芯片和三维生物打印4种3D细胞培养模型的构建方法,以及其在食源性致病菌中的研究进展和未来方向。该文将为食源性致病菌感染肠道相关基础研究和应用研究中最优模型的选择提供科学参考。In vitro intestinal models are important tools for studying the interactions between foodborne pathogens and their hosts.The traditional two-dimensional intestinal cell models have been the most commonly used in vitro models,but they have inherent limitations due to their inability to effectively simulate the physiological characteristics of the human intestine.The novel three-dimensional(3D)intestinal cell models can better simulate the microstructure,physiological functions,and microenvironment of the human intestine.They have gradually become powerful tools for studying intestinal physiology and pathology,gut microbiota,and pathogen-host interactions.This paper first introduces the basic structure of the intestine and the characteristics of the primary cell subtypes.It then analyzes and discusses the interactions between typical foodborne pathogens and the host.Finally,it reviews and discusses the construction methods of four types of 3D cell culture models,including multicellular spheroids,intestinal organoids,organ-on-a-chip,and 3D bioprinting,highlighting their research progress and potential future directions in the study of foodborne pathogens.This paper aims to provide a scientific reference for selecting the optimal models in basic and applied research related to intestinal infections by foodborne pathogens.

关 键 词：肠道 食源性致病菌 三维细胞模型 肠道类器官 器官芯片 

分 类 号：TS201.3[轻工技术与工程—食品科学]