非生物催芽花生白藜芦醇富集及对HEK293T细胞的保护作用  

Resveratrol Enrichment in Abiotic Sprouted Peanuts and Its Protective Effect on HEK293T Cells

作　　者：孙畅 徐浩然 秦凤贤 吕呈蔚 李倬林[1] 刘建 李铁柱[1] SUN Chang;XU Haoran;QIN Fengxian;LÜ Chengyu;LI Zhuolin;LIU Jian;LI Tiezhu(Institute of Agricultural Products Processing,Jilin Academy of Agricultural Sciences,Changchun 130033)

机构地区：[1]吉林省农业科学院农产品加工研究所,长春130033

出　　处：《中国食品学报》2025年第2期70-78,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：吉林省科技发展计划项目(20240601088RC);吉林省创新创业人才项目(第19批)(2023QN11);2024年度吉林省中医药科技项目(2024129,2024130)。

摘　　要：目的:探究非生物诱导催芽对花生中白藜芦醇(RES)富集的影响,并测定在最优富集条件下RES对HEK293T细胞氧化损伤的保护作用。方法:以光照种类、超声强度、硫酸铜浓度、发芽时间为单因素条件诱导花生发芽,根据单因素实验和正交试验探究非生物诱导催芽对RES的含量影响以及最优富集条件。通过细胞活力测定,得到RES对HEK293T细胞的最高毒性。通过检测细胞内活性氧(ROS)含量、细胞超氧化物歧化酶(SOD)活力和过氧化氢酶(CAT)活力,探究RES对H_(2)O_(2)诱导的HEK293T细胞的抗氧化损伤的保护效果。结果:光照、超声、硫酸铜都有促进发芽花生中白藜芦醇含量的效果,复合催芽对白藜芦醇富集呈促进作用。最佳花生芽白藜芦醇富集参数为:蓝光照射、超声强度120 W/cm^(3)、CuSO_(4)浓度0.6 mmol/L、发芽3 d,此时白藜芦醇含量为35.79μg/g。RES干预处理可以明显恢复H_(2)O_(2)诱导产生氧化损伤的HEK293T细胞活力,减少细胞内ROS的生产,增加SOD、CAT的活力。结论:光照等多重催芽可以富集发芽花生中白藜芦醇,RES对细胞有明显的氧化应激损伤保护作用。Objective:To investigate the effect of abiotic induction of germination on the enrichment of resveratrol(RES)in peanut,and to determine the protective effect of RES on the oxidative damage of HEK293T cells under the optimal enrichment conditions.Methods:Light type,ultrasound intensity,copper sulphate concentration and germination time were used as univariate conditions to induce peanut germination,and the effects of abiotic induction of germination on RES and the optimal enrichment conditions were investigated based on univariate and orthogonal experiments.The highest toxicity of RES on HEK293T cells was determined by cell viability assay.The protective effect of RES against H_(2)O_(2)-induced antioxidant damage in HEK293T cells was investigated by measuring intracellular reactive oxygen species(ROS)content,cellular superoxide dismutase(SOD)activity and catalase(CAT)activity.Results:Light,ultrasound and copper sulphate were all effective in promoting resveratrol content in germinated peanuts,and the combined germination showed a promoting effect on resveratrol enrichment.The optimal parameters for resveratrol enrichment in peanut buds were:Blue light irradiation,ultrasound intensity of 120 W/cm^(3),CuSO_(4) concentration of 0.6 mmol/L,and germination for 3 d.At this time,the resveratrol content of peanut buds was 35.79μg/g.RES intervention treatment significantly restored the viability of H_(2)O_(2)-induced oxidative damage-producing HEK293T cells,reduced the production of intracellular ROS,and increased the viability of SOD,CAT viability.Conclusion:Multiple germination such as light could enrich resveratrol in germinated peanuts,and RES has a significant protective effect on cells against oxidative stress damage.

关 键 词：非生物诱导催芽 花生 白藜芦醇 氧化应激损伤 

分 类 号：TS201.4[轻工技术与工程—食品科学]