模拟胃、肠消化日本沼虾原肌球蛋白稳定性比较  

Comparison of Stability of Macrobrachium nipponense Tropomyosin in Simulated Gastrointestinal Digestion

作　　者：陈娇[1,2,3] 华希玮 刘鑫 郑双艳[2] 陈红兵 谢彦海[1,2] CEHN Jiao;HUA Xiwei;LIU Xin;ZHENG Shuangyan;CHEN Hongbing;XIE Yanhai(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047;Sino-German Joint Research Institute,Nanchang University,Nanchang 330047;College of Food Science&Technology,Nanchang University,Nanchang 330047)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室,南昌330047 [2]南昌大学中德联合研究院,南昌330047 [3]南昌大学食品学院,南昌330047

出　　处：《中国食品学报》2025年第2期105-114,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(32060584);江西省自然科学基金项目(20232BAB205084)。

摘　　要：为比较成人和婴幼儿胃、肠消化对日本沼虾原肌球蛋白消化稳定性的影响差异,首先构建成人和婴幼儿体外静态消化模型,对原肌球蛋白(TM)进行体外消化。随后,用Tricine-SDS-PAGE、RP-HPLC和MALDI-TOF-MS等方法测定经体外模型模拟消化后TM的水解度和酸溶性肽浓度以及胃、肠消化产物的分子质量分布等。结果显示:模拟成人、婴幼儿胃消化终阶段的TM水解度分别为(17.99±1.41)%,(49.46±1.02)%;模拟成人、婴幼儿肠消化终阶段的TM水解度分别为(9.92±1.50)%,(30.68±1.04)%。模拟成人、婴幼儿胃消化终阶段的酸溶性肽质量浓度分别为(74.77±6.84),(46.39±7.5)μg/mL、;模拟成人、婴幼儿肠消化终阶段的酸溶性肽质量浓度分别为(303.99±12),(175.93±11.53)μg/mL。模拟成人胃、肠消化终阶段时,TM的消化片段主要集中分布于15~30 ku范围和20 ku以下,而模拟婴幼儿胃、肠最终的消化片段以大分子为主,主要在20 ku以上,说明成人模拟消化液的消化能力较婴幼儿模拟消化液消化能力强,TM易被成人消化而婴幼儿对TM的消化不完全,可能更容易引起过敏反应的发生。成人和婴幼儿胃肠消化的差异可能是由于成人的消化系统健全,而婴幼儿胃肠功能发育不健全、消化酶量较少造成的。In order to compare the effects of gastric and intestinal digestion on the digestive stability of Macrobrachium nipponense in adults and infants,static digestion models of adult and infants were constructed to digest tropomyosin(TM)in vitro.Then,Tricine-SDS-PAGE,RP-HPLC and MALDI-TOF-MS were used to determine the degree of hydrolysis of TM,the concentration of acid-soluble peptide and the molecular weight distribution of digestive products in stomach and intestine after the simulated digestion in vitro.The results showed that the hydrolysis degree of TM in the final stage of gastric digestion in simulated adults and infants was(17.99±1.41)%and(49.46±1.02)%,respectively.The degrees of TM hydrolysis in the final stage of intestinal digestion in simulated adults and infants were(9.92±1.50)%and(30.68±1.04)%,respectively.The mass concentrations of acid-soluble peptides at the final stage of gastric digestion in simulated adults and infants were(74.77±6.84)μg/mL and(46.39±7.5)μg/mL,respectively.The mass concentrations of acid-soluble peptides at the final stage of intestinal digestion in simulated adults and infants were(303.99±12)μg/mL and(175.93±11.53)μg/mL,respectively.In the final stage of simulated adult stomach and intestine digestion,the digestive fragments of TM were mainly distributed in the range of 15-30 ku and below 20 ku,while the final digestive fragments of simulated infant stomach and intestine were mainly macromolecules,mainly above 20 ku,indicating that the digestive capacity of adult simulated digestive fluid was stronger than that of infant simulated digestive fluid.TM was easily digested by adults,while infants and young children had incomplete digestion of TM,which might be more likely to cause allergic reactions.The difference in gastrointestinal digestion between adults and infants might be due to the fact that adults had a sound digestive system,while infants had less developed gastrointestinal function and low amount of digestive enzymes.

关 键 词：成人过敏 婴幼儿过敏 原肌球蛋白 体外模拟胃肠消化 消化稳定性 

分 类 号：R72[医药卫生—儿科]