具有潜在降压功能益生乳酸菌的筛选及其特性的研究  

Screening and Characterization of Probiotic Lactic Acid Bacteria with Potential Antihypertensive Function

作　　者：王睿敏 郑冬艳 但霞 李仁芳 曾庆坤 吴凤娇 黄丽 李玲 WANG Ruimin;ZHENG Dongyan;DAN Xia;LI Renfang;ZENG Qingkun;WU Fengjiao;HUANG Li;LI Ling(Guangxi Buffalo Research Institute,Nanning 530001,China;College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,China;Guangxi Royal Dairy Group Co.,Ltd.,Nanning 530007,China)

机构地区：[1]广西壮族自治区水牛研究所,广西南宁530001 [2]广西中医药大学药学院,广西南宁530001 [3]广西皇氏乳业有限公司,广西南宁530007

出　　处：《食品科技》2025年第1期1-8,共8页Food Science and Technology

基　　金：广西重点研发计划项目(桂科AB20297024);南宁市创新创业领军人才“邕江计划”项目(2021018);国家现代农业产业技术体系广西创新团队建设项目(nycytxgxcxtd-2021-21-02);广西农业科技自筹经费项目(Z202049)。

摘　　要：以41株乳酸菌作为研究对象,以γ-氨基丁酸(γ-Aminobutyric acid,GABA)和血管紧张素转换酶(Angiotensin converting enzyme,ACE)抑制活性为主要指标筛选获得具有降压功能的益生菌株,并分析其活菌数、pH值、滴定酸度以及耐酸性、耐胆盐能力等特性。结果表明,从41株乳酸菌中筛选出4株GABA含量高且ACE抑制率高的菌株,分别为干酪乳酪杆菌K8和SLK30、美洲虎乳酪杆菌K44、植物乳植物杆菌E44。4株菌株活菌数均大于10~6 CFU/mL,4株菌株凝乳pH值范围在4.54~4.69之间,酸度均在82?T以上。在模拟胃酸环境中,当pH值在2.0~3.0范围内时,4株乳酸菌的存活率均大于65%;当胆盐浓度在0~1.0%范围内时,均具有较好的耐受性,它们的存活率与胆盐浓度呈负相关。In this study,41 strains of lactic acid bacteria were selected as the research objects,and the inhibitory activities ofγ-aminobutyric acid(GABA)and angiotensin converting enzyme(ACE)were used as the main indicators to screen and obtain probiotics with the function of lowering blood pressure.The fermentation characteristics such as the number of viable bacteria,pH value,titratable acidity,as well as the probiotic characteristics such as acid resistance,bile salt resistance were also analyzed.The results showed that four strains with high GABA content and high ACE inhibition were screened out from 41Lactobacillus strains,namely Lacticaseibacillus casei K8 and SLK30,Lacticaseibacillus pantheris K44,and Lactiplantibacillus plantarum E44.The viable bacterial counts of the four strains were all greater than 10~6 CFU/mL,and the curd pH of the four strains ranged from 4.54 to 4.69,the acidity was above 82?T.In the simulated gastric acid environment,the survival rate of all four strains of lactic acid bacteria was greater than 65%when the pH was in the range of 2.0 to 3.0.All of them were well tolerated when the bile salt concentration was in the range of 0 to 1.0%,and their survival rates were negatively correlated with the bile salt concentration.

关 键 词：乳酸菌 降压功能 血管紧张素转换酶 Γ-氨基丁酸 发酵乳 

分 类 号：TS201.3[轻工技术与工程—食品科学]