Dietary carbohydrate to lipid ratio affects growth,reproductive performance and health of female yellow catfish(Pelteobagrus fulvidragrus):A lipidomics analysis  

作　　者：Shuzhan Fei Zheng Chen Haokun Liu Junyan Jin Yunxia Yang Dong Han Xiaoming Zhu Shuoqi Xie 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China [2]College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100049,China [3]Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,Guangdong 510380,China [4]Hubei Hongshan Laboratory,Wuhan 430070,China [5]The Innovative Academy of Seed Design,Chinese Academy of Sciences,Wuhan 430072,China

出　　处：《Animal Nutrition》2024年第4期429-441,共13页动物营养(英文版)

基　　金：supported by the National Key Research and Development Programof China(2022YFD2400900);the earmarked fund for China Agriculture Research System(CARS-46).

摘　　要：This study aimed to examine the impact of dietary carbohydrate to lipid(CHO/L)ratio on the growth,reproductive,and offspring performance of broodstock yellow catfish,and to elucidate the metabolic differences between mothers and offspring using lipidomics.Five isonitrogenous and isoenergetic diets with varying CHO/L ratios(0.65,1.44,2.11,3.13,and 5.36)were fed to five groups of female broodfish with three replicates per group and 35 female broodfish per replocate in a pond-cage culture system.After an eight-week feeding trial,the dietary CHO/L ratio had a significant impact on the growth and reproductive performance of female yellow catfish.The weight gain ratio(WGR)and specific growth rate(SGR)in the CHO/L0.65 and CHO/L2.11 groups were significantly higher than those in the CHO/L5.36 group(P<0.05).The fertilization and hatching rates were the highest when the dietary CHO/L ratio was 0.65 and 2.11,respectively.When the dietary CHO/L ratio was 3.13 and 5.36,the plasma contents of testosterone(T)was significantly lower than those of other groups(P=0.013),and the plasma vitellogenin(VTG)content was the lowest when the CHO/L ratio was 5.36.The plasma contents of estradiol(E2)significantly decreased with increasing dietary CHO/L ratio(P_(L)=0.012).Lipidomic analysis revealed that the ovary primarily consisted of five subclasses in terms of lipid composition,namely triglyceride,fatty acyl,sterol,glycerophospholipid,and sphingolipid;however,sphingolipids were not detected in the larvae.The relative expression levels of the ovarian lipid metabolism-related genes sterol regulatory element binding protein 1(srebp1),acetyl-CoA carboxylase(acc),delta(12)-oleate desaturase(fad2),and elongation of very long chain fatty acids protein 5(elvol5)significantly increased with increasing dietary CHO/L ratio(P<0.05).The relative expression levels of lipid metabolism-related genes srebp 1,peroxisome proliferator activated receptor a(ppara),carnitine palmitoyl transferase 1 isoform(cpt),adipose triglyceride lipase(atgl),fad2,and elvol5

关 键 词：Yellow catfish CARBOHYDRATE LIPID Reproductive performance LIPIDOMICS 

分 类 号：R73[医药卫生—肿瘤]