山楂果胶寡糖对过氧化氢诱导的人胚肺成纤维细胞衰老的作用  

Effect of Pectin Oligosaccharides from Hawthorn on the H2O2-induced Human Embryonic Lung Fibroblast Cells

作　　者：李涛 纪文华 董红敬 王珍强 黄群 王晓 LI Tao;JI Wenhua;DONG Hongjing;WANG Zhenqiang;HUANG Qun;WANG Xiao(Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products,Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,School of Pharmaceutical Sciences,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;School of Pharmaceutical Sciences,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Guizhou Ecological Food Creation Engineering Technology Center,Guizhou Medical University,Guian New Area 550025,China)

机构地区：[1]齐鲁工业大学(山东省科学院),山东省分析测试中心,天然产物分离提取共性技术创新与应用山东省工程研究中心,山东济南250014 [2]齐鲁工业大学(山东省科学院)药学院,山东省高等学校天然药物活性成分研究重点实验室,山东济南250014 [3]山东中医药大学药学院,山东济南250355 [4]贵州医科大学,贵州省生态食品创制工程技术中心,贵州贵安新区550025

出　　处：《食品工业科技》2025年第7期346-354,共9页Science and Technology of Food Industry

基　　金：“十四五”国家重点研发计划(2023YFC3503805);泰山学者工程(NO.tsqnz20231230);济南市创新团队(202228020)。

摘　　要：以过氧化氢(H2O2)诱导的人胚肺成纤维细胞(Human embryonic lung fibroblast cells,MRC-5)作为衰老模型,通过对细胞活力、存活率、β-半乳糖苷酶活性、线粒体膜电位、活性氧(ROS)含量、抗氧化能力和p53/p21/p16信号通路相关蛋白的表达水平等指标进行测定,研究山楂果胶寡糖(HPOS)对MRC-5衰老细胞的抗衰老作用。结果表明:与模型组相比,在浓度为0.8 mg/mL的山楂果胶寡糖干预下,MRC-5衰老细胞的细胞活力、存活率和线粒体膜电位分别极显著增加了16.4%,17.0%和284.3%(P<0.01);β-半乳糖苷酶阳性细胞比例和ROS荧光强度分别极显著降低了66.9%、77.2%(P<0.01);超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力较模型组细胞分别增加了72.0%和50.0%,而丙二醛(MDA)含量极显著降低了42.6%(P<0.01)。浓度为0.8 mg/mL的山楂果胶寡糖干预极显著抑制了p53/p21/p16信号通路中p53、p21和p16蛋白的表达(P<0.01)。总之,山楂果胶寡糖通过抑制由衰老诱发的细胞凋亡和氧化应激损伤,调节p53/p21/p16通路,从而改善H2O2诱导的MRC-5细胞衰老。本研究结果将为开发山楂果胶寡糖作为抗衰老的功能性食品配料提供科学依据。In this study,the anti-aging effects and mechanism of action of pectin oligosaccharides from hawthorn(HPOS)on H2O2-induced human embryonic lung fibroblast cells were evaluated using the MRC-5 cell line.The effects on cell viability,survival rate,β-galactosidase activity,mitochondrial membrane potential,the content of reactive oxygen species(ROS),antioxidant capacity,and the expression levels of proteins associated with the p53/p21/p16 signaling pathway were evaluated.The findings illustrated that compared with the model group,under the intervention of HPOS at a concentration of 0.8 mg/mL,the cell viability,survival rate,and mitochondrial membrane potential of MRC-5 cells increased by 16.4%,17.0%,and 284.3%(P<0.01),respectively.Furthermore,the proportion ofβ-galactosidase-positive cells and fluorescence intensity of ROS significantly decreased by 66.9%,77.2%(P<0.01),respectively.The superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)contents increased by 72.0%and 50.0%,respectively.Compared to that in the model group,the malondialdehyde(MDA)content decreased by 42.6%(P<0.01)significantly.HPOS at a concentration of 0.8 mg/mL significantly inhibited p53,p21,and p16 expression in the p53/p21/p16 pathway(P<0.01).Thus,HPOS ameliorates the H2O2-induced aging of MRC-5 cells by inhibiting apoptosis and oxidative stress damage along with regulating the p53/p21/p16 pathway,thereby providing a scientific basis for the development of HPOS as a functional food ingredient that acts against aging.

关 键 词：山楂果胶寡糖 衰老 细胞凋亡 抗氧化能力 p53/p21/p16通路 

分 类 号：TS201.4[轻工技术与工程—食品科学]