不同时期烟草叶片挥发物释放规律研究  

Research on the Release Regularity of Volatile Compounds in Tobacco Leaves at Different Growth Stages

作　　者：池琴 母银林[3] 胡恩明 李春燕 王道平 CHI Qin;MU Yinlin;HU Enming;LI Chunyan;WANG Daoping(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China;Guizhou University,Guiyang 550014,China)

机构地区：[1]贵州医科大学,省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014 [2]贵州省天然产物研究中心,贵州贵阳550014 [3]贵州大学,贵州贵阳550014

出　　处：《云南化工》2025年第3期30-36,共7页Yunnan Chemical Technology

基　　金：黔科合基础-ZK[2021]一般534。

摘　　要：探究不同时期烟草叶片挥发物释放规律,对于提升烟草品质、培育高品质的烟草品种具有重大意义。研究采用顶空固相微萃取和气相色谱-质谱联用技术,通过2 cm-50/30μm DVB/CAR/PDMS StableFlex纤维头吸附不同时期烟草叶片挥发物,系统研究了烟草生长过程中不同时期的挥发物成分。从烟草团棵期烟叶中分离鉴定出24种化合物,烟草旺长期烟叶中分离鉴定出64种化合物,烟草成熟期烟叶中分离鉴定出43种化合物,烤干烟烟叶中分离鉴定出75种化合物。通过分析对比不同时期的挥发物成分,发现团棵期和旺长期烟叶中各类物质变化规律一致,成熟期和烤干烟烟叶中各类物质变化规律一致,烤干烟烟叶中特有的36类化合物可能与其香味特征的形成密切相关。To explore the release patterns of volatiles in tobacco leaves at different periods is of great significance for improving the quality of tobacco and cultivating high quality tobacco varieties.In this study,headspace solid phase microextraction(HS-SPME)and gas chromatography mass spectrometry(GC-MS)were employed.The volatiles in tobacco leaves at different periods were adsorbed by 2 cm-50/30μm DVB/CAR/PDMS StableFlex fiber heads,and the volatile components at different stages during the growth process of tobacco were systematically studied.24 compounds were identified from the rosette stage of tobacco,and 64 compounds,43 compounds and 75 compounds were identified from the vigorous growth stage,the maturity stage and the cured tobacco,respectively.Through the analysis and comparison of the volatile components at different periods,it was found that the change patterns of various components were consistent in tobacco leaves between the rosette and the vigorous growth stage,and that of components between the maturity stage and the cured tobacco stage are also consistent.The 36 types of compounds unique to the cured tobacco may be closely related to the formation of its flavor characteristics.

关 键 词：烟叶 烟草挥发物 固相微萃取 气相色谱-质谱 不同时期 释放规律 香味特征 

分 类 号：TS411[农业科学—烟草工业]