香菇多糖金属离子对其结构和体内抗氧化活性的影响  

Effects of Removal of Metal Ions Bound to Lentinan on Its Structure and in Vivo Antioxidant Activity

作　　者：王慧[1] 王岸娜[1] 董赵微 朱俊友 WANG Hui;WANG Anna;DONG Zhaowei;ZHU Junyou(School of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001

出　　处：《食品科学》2025年第7期119-124,共6页Food Science

基　　金：河南工业大学博士基金项目(2018BS076);河南省重点研发专项(221111112000)。

摘　　要：本实验利用乙二胺四乙酸二钠螯合脱除香菇多糖中的内源性金属离子,研究脱除金属离子香菇多糖的理化性质、结构和抗氧化活性变化。分别用硫酸-苯酚法、考马斯亮蓝法和间羟基联苯法检测脱金属离子前后香菇多糖总糖、蛋白质和糖醛酸含量,并通过高效凝胶渗透色谱法、离子色谱法、扫描电镜和X射线衍射技术分析香菇多糖分子质量、单糖组成和高级结构。同时利用动物实验测定脱除金属离子前后香菇多糖的体内抗氧化活性。结果表明,脱除金属离子后香菇多糖的总糖含量和糖醛酸含量升高,相对分子质量范围和分布发生变化,单糖组成不变,但比例发生改变。脱除金属离子后,香菇多糖表面结构变得松散,螺旋结构发生改变。小鼠血清抗氧化实验结果表明,脱除金属离子后,香菇多糖的体内抗氧化活性降低。综上,金属离子脱除会破坏香菇多糖结构并使其体内抗氧化活性减弱。Ethylenediaminetetraacetic acid disodium(EDTA-2Na)was used as a chelating agent to remove metal ions bound to lentinan(LNT).Changes in physicochemical properties,structure and antioxidant activity of LNT were studied after the removal of metal ions.The total sugar,protein and uronic acid contents were determined by the phenol-sulfuric acid method,Bradford’s method and the m-hydroxydiphenyl method,respectively.High performance gel permeation chromatography(HPGPC),ion chromatography(IC),scanning electron microscopy(SEM)and X-ray diffraction(XRD)were used to determine the relative molecular mass,monosaccharide composition and advanced structure of LNT and demetallized LNT(dLNT),respectively.The in vivo antioxidant activities of LNT and dLNT were evaluated in mice.The results showed that after the removal of metal ions,the content of total sugar and uronic acid of LNT increased,the molecular mass range and distribution changed,and the composition but not proportion of monosaccharides remained unchanged.After metal ion removal,the surface structure became loose and the helical structure changed.Moreover,the antioxidant activity of dLNT in mouse serum was decreased compared with that of LNT.In summary,the removal of metal ions could destroy the structure of lentinan and weaken its antioxidant activity.

关 键 词：香菇多糖 金属离子 结构 体内抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]