产酯酶和酯类化合物的粟酒裂殖酵母筛选及应用  

Screening and Application of Schizosaccharomyces pombe with Production Esterases and Ester Compounds

作　　者：罗浩 但俊林 蔡岭肸 刘杰 邹伟 LUO Hao;DAN Junlin;CAI Lingxi;LIU Jie;ZOU Wei(School of Biological Engineering,Sichuan University of Science&Engineering,Yibin 644005,Sichuan,China;Anhui Linshui Liquor Co.,Ltd.,Lu´an 237471,Anhui,China;Liquor Making Biotechnology and Application Key Laboratory of Sichuan Province,Sichuan University of Science&Engineering,Yibin 644005,Sichuan,China;Liquor Brewing Biotechnology and Intelligent Manufacturing Key Laboratory of China Light Industry,Sichuan University of Science&Engineering,Yibin 644005,Sichuan,China)

机构地区：[1]四川轻化工大学生物工程学院,四川宜宾644005 [2]安徽临水酒业有限公司,安徽六安237471 [3]四川轻化工大学酿酒生物技术及应用四川省重点实验室,四川宜宾644005 [4]四川轻化工大学中国轻工业酿酒生物技术及智能制造重点实验室,四川宜宾644005

出　　处：《食品研究与开发》2025年第6期152-160,共9页Food Research and Development

基　　金：酿酒生物技术及应用四川省重点实验室项目(NJ2023⁃07);中国轻工业酿酒生物技术及智能制造重点实验室项目(2023⁃05);四川轻化工大学校级大学生创新创业训练计划项目(CX2023133)。

摘　　要：酯化液在改善和提升食品口感和风味方面拥有较大的市场发展潜力,并受到社会的普遍关注。该文从浓香型白酒大曲中进行产酯酵母菌的筛选研究,得到一株产酯酶酶活力为(15.52±0.03)U/mL的菌株JM2-5。该菌株的透明圈直径与菌落直径比值为2.57±0.03,经分子生物学鉴定为粟酒裂殖酵母(Schizosaccharomyces pombe)。对其形态、生理耐受性及发酵性能进行研究,结果发现在不添加乙醇的情况下,该菌在30℃厌氧发酵9 d能够自身合成乙酸乙酯631.000 mg/L、乳酸乙酯86.000 mg/L、己酸乙酯25.000 mg/L。此外,S.pombe JM2-5菌株产生的酯酶具有体外催化产生高含量酯类物质的能力。综上,S.pombe JM2-5菌株可应用于白酒,以促进生产过程中的酯化反应;或者作为催化剂,通过催化酯化反应产物的产生,应用于调味料酒的生产。Esterification liquids have great market development potential in improving the taste and flavor of food,and have caught widespread attention from society.This paper conducted a study on screening ester-producing yeasts from strongflavor Baijiu Daqu and obtained a strain JM2-5 with the esterase enzyme activity of(15.52±0.03)U/mL.The ratio of the transparent ring diameter to the colony diameter of this strain was 2.57±0.03,and it was identified as Schizosaccharomyces pombe by molecular biological techniques.Meanwhile,the morphology,physiological tolerance,and fermentation performance of JM2-5 were studied.The results showed that without the addition of ethanol,the strain was able to synthesize 631.000 mg/L of ethyl acetate,86.000 mg/L of ethyl lactate,and 25.000 mg/L of ethyl hexanoate during anaerobic fermentation at 30℃for nine days.Additionally,the esterase produced by the S.pombe JM2-5 strain could catalyze the production of high-content esters in vitro.In summary,the S.pombe JM2-5 strain can be employed in Baijiu production to promote the esterification reaction during production or can be adopted as a catalyst to catalyze the esterification reaction product to produce flavoring liquor.

关 键 词：粟酒裂殖酵母 酯酶 己酸乙酯 白酒 调味液 

分 类 号：TS2[轻工技术与工程—食品科学与工程]