顶空箭形固相微萃取-气相色谱-质谱法测定食盐中9种短链脂肪酸  

Determination of 9 kinds of short-chain fatty acids in salt by headspace solid phase microextraction arrow-gas chromatography-mass spectrometry

作　　者：徐婧婧 吴肖肖 蒋迪尧 张驰 褚兰玲 纪晗旭 XU Jing-Jing;WU Xiao-Xiao;JIANG Di-Yao;ZHANG Chi;CHU Lan-Ling;JI Han-Xu(Key Laboratory of Biotoxin Analysis&Assessment,State Administration for Market Regulation,Nanjing Institute of Product Quality Inspection(Nanjing Institute of Quality Development and Advanced Technology Application),Nanjing 210019,China;College of Light Industry and Food,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]南京市产品质量监督检验院(南京市质量发展与先进技术应用研究院),国家市场监督管理总局重点实验室(生物毒素分析与评价),南京210019 [2]南京林业大学轻工与食品学院,南京210037

出　　处：《食品安全质量检测学报》2025年第7期248-254,共7页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2023YFF1105102);国家市场监管总局科技项目(2022MK160);江苏省科协青年科技人才托举工程项目(JSTJ-2023-XH007)。

摘　　要：目的建立顶空箭形固相微萃取-气相色谱-质谱法测定食盐中短链脂肪酸的定性和定量分析方法。方法在食盐样品中加入磷酸溶液,基于箭形固相微萃取技术对短链脂肪酸进行萃取富集,探究了萃取头、萃取温度、萃取时间、孵育时间对萃取效果的影响,目标物用气相色谱-质谱法检测,外标法定量。结果萃取头100μm Polyacrylate对短链脂肪酸有较好的选择性,在萃取温度40℃、时间30 min,解析时间5 min,孵育时间20 min的条件下,9种短链脂肪酸线性关系良好,相关系数大于0.995,检出限为0.25~25.00μg/kg,回收率为92.2%~100.1%,相对标准偏差为1.4%~9.4%。结论本研究建立的食盐中短链脂肪酸的分析方法样品制备简单,灵敏度高,回收率和稳定性好,适用于食盐中短链脂肪酸的检测。Objective To establish a qualitative and quantitative analytical method for determination of short-chain fatty acids in table salt using headspace solid phase microextraction arrow-gas chromatography-mass spectrometry.Methods A phosphoric acid solution was added to the salt samples,and the headspace solid phase microextraction arrow technique was employed to extract and enrich the short-chain fatty acids.The effects of extraction fiber,extraction temperature,extraction time,and incubation time on the extraction efficiency were investigated.The target compounds were detected by gas chromatography-mass spectrometry and quantified using the external standard method.Results The 100μm Polyacrylate extraction fiber demonstrated good selectivity for short-chain fatty acids.Under optimal conditions(extraction temperature of 40°C,extraction time of 30 min,desorption time of 5 min and incubation time of 20 min),the method showed excellent linearity for 9 kinds of short-chain fatty acids,with correlation coefficients greater than 0.995.The limits of detection ranged from 0.25 to 25.00μg/kg,the recovery rates were between 92.2%and 100.1%,and the relative standard deviations ranged from 1.4%to 9.4%.Conclusion The established method for analyzing short-chain fatty acids in table salt is characterized by simple sample preparation,high sensitivity,good recovery rates and stability,making it suitable for the detection of short-chain fatty acids in table salt.

关 键 词：食盐 短链脂肪酸 顶空固相微萃取 气相色谱-质谱法 

分 类 号：R28[医药卫生—中药学]