Aloin ameliorates cecal ligation and puncture-induced sepsis in mice by attenuating inflammation and modulating gut microbiota  

作　　者：Jingqian Su Jianbin Xiao Siyuan Chen Heng Zhao Xiaoni Zhang Zhihua Feng Kunsen Chen Biyun Guan Wenzhi Chen Youqiang Chen Duo Chen Qi Chen 

机构地区：[1]Fujian Key Laboratory of Innate Immune Biology,Biomedical Research Center of South China,College of Life Science,Fujian Normal University,Fuzhou 350117,China [2]The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration,Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization,Southern Institute of Oceanography,College of Life Science,Fujian Normal University,Fuzhou 350117,China

出　　处：《Food Science and Human Wellness》2025年第2期550-568,共19页食品科学与人类健康(英文)

基　　金：supported by the National Natural Science Foundation of China(81803547);the Natural Science Foundation of Fujian Province,China(2021J01204);Fujian Provincial Regional Development Project(2021N3005)。

摘　　要：Most Aloe species are used as new food or functional food ingredient.Even though widely known for its health benefits,the anti-inflammatory effects and underlying mechanisms of Aloin(Alo),an anthraquinone compound isolated from plant species of the genus Aloe,remain unidentified.Here,we investigated the protective effects of Alo against cecal ligation and puncture(CLP)-induced sepsis and microflora in mice.Alo significantly improved CLP-induced sepsis and the survival rate of septic mice,downregulated the expression of proinflammatory factors,and decreased the infiltration of inflammatory cells in tissues.Alo upregulated the proportion of peritoneal macrophages,reduced the number of peritoneal bacteria,decreased the content of short-chain fatty acids and bile acids in the abdominal cavity,and suppressed Toll-like receptor(TLR)-2/4/nuclear factor kappa-B(NF-κB)/NOD-like receptor thermal protein domain associated protein 3(NLRP3)/Caspase-1/3/8 signaling.Furthermore,Alo altered the composition of the microbiome and promoted the growth of Lactobacillus,which showed a stronger anti-inflammatory effect.Whole-genome analysis identified the genes Saa3,Il10,Fpr1,and Eif4a1 associated with the protective effects of Alo in mice with CLP-induced sepsis.Overall,our results provide novel insights into the therapeutic potential and mechanism of action of Alo in the treatment of sepsis.

关 键 词：ALOIN Gut microbiome SEPSIS INFLAMMATION Cecal ligation and puncture 

分 类 号：R73[医药卫生—肿瘤]