Effect of L-theanine combined with geniposide on depression-like behavior induced by the chronic unpredictable mild stress(CUMS)in mice  

作　　者：Qiming Yu Yan Zhu Tao Jiang Ying Gao Nan Ling Na Wang John Shi Weichun Pan Qizhen Du Junfeng Yin 

机构地区：[1]Key Laboratory of Tea Biology and Resources Utilization,Ministry of Agriculture and Rural Affairs,Tea Research Institute of Chinese Academy of Agricultural Sciences,Hangzhou 310008,China [2]College of Public Health,Guilin Medical University,Guilin 541199,China [3]College of Food and Health Sciences,Zhejiang A&F University,Hangzhou 311300,China [4]Guelph Food Research Center,Agriculture and Agri-Food Canada,Guelph N1G 5C9,Canada [5]Zhejiang Gongshang University,Hangzhou 310018,China

出　　处：《Food Science and Human Wellness》2025年第4期1424-1440,共17页食品科学与人类健康(英文)

基　　金：funded by Innovation Project for Chinese Academy of Agricultural Sciences(CAAS-ASTIP-TRI);China Agriculture Research System of MOF and MARA(CARS-19)。

摘　　要：Depression is a prevalent mental disorder with limited effective treatments,posing a significant global issue.This study explored L-theanine and geniposide,key components in“food-medicine homology”materials,to determine if their combination(TG)could alleviate depression-like behaviors and hippocampal neuronal damage in a chronic unpredictable mild stress(CUMS)mouse model.Male C57BL/6J mice were divided into control,CUMS model,and CUMS+TG groups with varying doses.The CUMS group displayed depressionlike behaviors,including reduced activity and sucrose preference.TG treatment partially reversed these changes,significantly increasing antioxidant enzyme activities,decreasing pro-inflammatory cytokine levels,and improving neuromodulator levels.RNA-seq analysis identified the transthyretin(TTR)gene,upregulated in the model group but downregulated after TG treatment.TG treatment modulated intestinal microbiota composition compared to the CUMS group,including increased Firmicutes,reduced Bacteroidetes and Prevotella,and variable changes in Bifidobacterium abundance.In conclusion,our study indicates that CUMS exposure upregulates stress hormones and TTR expression,associated with neuroinflammation,oxidative stress,monoamine depletion,depression-like behaviors,and intestinal microbiota dysbiosis.TG treatment alleviates these effects and modulates intestinal microbiota,suggesting L-theanine and geniposide's potential as a novel depression therapy.

关 键 词：CUMS DEPRESSION L-THEANINE GENIPOSIDE TRANSTHYRETIN 

分 类 号：R749.4[医药卫生—神经病学与精神病学] TS201.4[医药卫生—临床医学]