Microbial fermentation of blue honeysuckle(Lonicera caerulea L.)polyphenol promotes insulin secretion and intervenes in obesity by promoting short-chain fatty acids production  

作　　者：Fangyi Pei Xinbo Cao Yanxin Ren Liangyang Mao Yueyi Liu Jingping Ge Wenxiang Ping 

机构地区：[1]Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education&Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region&Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region&Key Laboratory of Microbiology,College of Heilongjiang Province&School of Life Sciences,Heilongjiang University,Harbin 150080,China [2]Office of Academic Research,Qiqihar Medical University,Qiqihar 161006,China

出　　处：《Food Science and Human Wellness》2025年第4期1449-1468,共20页食品科学与人类健康(英文)

基　　金：supported by the Key Program of Heilongjiang Provincial Natural Science Foundation of China(ZD2020C008);the National Natural Science Foundation of China(32071519);the Heilongjiang University Graduate Innovation Research Project(YJSCX2022-031HLJU);the Qiqihar Science and Technology Plan Joint Guidance Project(LSFGG-2022037);Scientific Research Project of Ecological Environment Protection of Heilongjiang Provincial Department of Ecological Environment(HST2022TR004)。

摘　　要：During obesity treatment,clinicians have been burdened by the ever-increasing number of patients and the side effects of drug treatment.Finding new plant-derived active ingredients as an intervention for obesity has become a research focus.In this study,Plackett-Burman and Central Composite Design experiments,plant-wide target metabolomics technology,simulated human colon fermentation test,and cell experiment in vitro were successively used to investigate the effects of fermented and unfermented polyphenols(FP and UP,respectively)of blue honeysuckle on obesity.After the optimization of the response surface method,polyphenol content((1878.08±14.53)mg/100 g)increased by 2.63 times after Lactobacillus rhamnosus 6224 and Saccharomyces cerevisiae W5 mixed fermentation.There were 23 significantly different metabolites were observed in FP and UP.Of them,7 different metabolites were significantly and positively correlated with short-chain fatty acids(SCFAs),whereas negatively correlated with lipopolysaccharides(LPS).The contents of these 7 metabolites were significantly increased in FP.Furthermore,the effects of FP and UP on the SCFA biosynthesis pathway were different.Cell experiments revealed that polyphenols can significantly promote the secretion of gastrointestinal endogenous hormones and insulin and inhibit interleukin-6 levels.Linear regression analysis revealed SCFAs had a positive regulatory effect on glycolipid metabolism,whereas LPS had a negative effect.Overall,these crucial findings provide an economical and simple method for obtaining blue honeysuckle polyphenols and laying a theoretical foundation for its application in the therapeutic intervention against obesity.

关 键 词：OBESITY Blue honeysuckle POLYPHENOL Short-chain fatty acids In vitro fecal fermentation 

分 类 号：R58[医药卫生—内分泌]