Detecting plasma SHOX2, HOXA9, SEPTIN9, and RASSF1A methylation and circulating cancer cells for cholangiocarcinoma clinical diagnosis and monitoring  

作　　者：Jing Yu Qiu-Chen Liu Shuang-Yan Lu Shun Wang Hua Zhang 

机构地区：[1]Department of Laboratory,Wuhan Hospital of Traditional Chinese and Western Medicine(Wuhan's TCWM Hospital),Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China [2]Department of Laboratory,Sinopharm Dongfeng General Hospital,Hubei University of Medicine,Shiyan 442008,Hubei Province,China [3]Department of Laboratory,Hubei Cancer Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430079,Hubei Province,China [4]Department of Blood Transfusion,Wuhan Chinese and Western Medicine Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China [5]Department of Laboratory,Wuhan Chinese and Western Medicine Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2025年第4期210-222,共13页世界胃肠肿瘤学杂志(英文)

基　　金：Supported by the Medical Talents of Wuhan Health and Family Planning Commission,No.2017[51](to Yu J);the Medical Talents of Wuhan Hospital of Traditional Chinese and Western Medicine(to Yu J);the Hubei Natural Science Foundation,No.2023AFB1091;Wuhan Medical Research Project,No.WX23A36(to Yu J).

摘　　要：BACKGROUND Cholangiocarcinoma(CCA),also known as bile duct cancer,is a devastating malignancy primarily affecting the biliary tract.AIM To assess their performance in clinical diagnosis and monitoring of CCA,plasma methylation and circulating tumor cells were detected.METHODS Plasma samples were collected from Hubei Cancer Hospital(n=156).Plasma DNA was tested to detect SHOX2,HOXA9,SEPTIN9,and RASSF1A methylation using TaqMan PCR.Circulating tumor cells(CTCs)were detected in the peripheral blood of patients using the United States Food and Drug Administration-approved cell search system before and after clinical therapy.The CCA diagnostic value was estimated using the area under the curve.The independent prognosis risk factors for patients with CCA were estimated using Cox and logistic regression analyses.RESULTS The sensitivity and specificity of the four DNA plasma methylations exhibited 64.74%sensitivity and 93.88%specificity for detecting CCA.The receiver operating characteristic curve of the combined value for CCA diagnosis in plasma was 0.828±0.032.RASSF1A plasma methylation was related to the prognosis of patients with CCA.We determined the prognostic hazard ratio for CCA using CTC count,tumor stage,methylation,and carbohydrate antigen 19-9 levels as key factors.Our overall survival nomogram achieved a C-index of 0.705(0.605-0.805).CONCLUSION SHOX2,HOXA9,SEPTIN9,and RASSF1A plasma methylation demonstrated increased sensitivity for diagnosing CCA.RASSF1A plasma methylation and CTCs were valuable predictors to assess CCA prognosis and recurrence.

关 键 词：CHOLANGIOCARCINOMA METHYLATION Circulating cancer cells Diagnosis PROGNOSIS 

分 类 号：R73[医药卫生—肿瘤]