Snail family transcriptional repressor 1 radiosensitizes esophageal cancer via epithelial-mesenchymal transition signaling: From bioinformatics to integrated study  

作　　者：Xiao-Li Lv Qi-Liang Peng Xin-Peng Wang Zhi-Chao Fu Jian-Ping Cao Jian Wang Li-Li Wang Yang Jiao 

机构地区：[1]State Key Laboratory of Radiation Medicine and Protection,School of Radiation Medicine and Protection,Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions,Soochow University,Suzhou 215123,Jiangsu Province,China [2]Department of Radiotherapy,The First Affiliated Hospital of Soochow University,Suzhou 215006,Jiangsu Province,China [3]Department of Radiotherapy and Oncology,The Second Affiliated Hospital of Soochow University,Suzhou 215004,Jiangsu Province,China [4]Department of Radiotherapy,Fuzong Clinical Medical College of Fujian Medical University,Fuzhou 350112,Fujian Province,China [5]Department of Radiotherapy,The Affiliated Jiangyin People’s Hospital of Nantong University,Jiangyin 214400,Jiangsu Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2025年第4期286-299,共14页世界胃肠肿瘤学杂志(英文)

基　　金：Supported by the National Key R&D Program of China,No.2022YFC2503700 and No.2022YFC2503703;the National Health Commission Key Laboratory of Nuclear Technology Medical Transformation(Mianyang Central Hospital),No.2023HYX005.

摘　　要：BACKGROUND Esophageal cancer(ESCA)poses a significant challenge in oncology because of the limited treatment options and poor prognosis.Therefore,enhancing the therapeutic effects of radiotherapy for ESCA and identifying relevant therapeutic targets are crucial for improving both the survival rate and quality of life of patients.AIM To define the role of the transcription factor Snail family transcriptional repressor 1(SNAI1)in ESCA,particularly its regulation of radiosensitivity.METHODS A comprehensive analysis of TCGA data assessed SNAI1 expression in ESCA.Survival curves correlated SNAI1 levels with radiotherapy outcomes.Colony formation assays,flow cytometry,and a xenograft model were used to evaluate tumor radiosensitivity and apoptosis.Western blot validated protein expression,while Chromatin im-munoprecipitation assays examined SNAI1's role in regulating epithelial-mesenchymal transition(EMT).RESULTS SNAI1 expression in ESCA cell lines and clinical specimens emphasizes its central role in this disease.Elevated SNAI1 expression is correlated with unfavorable outcomes in radiotherapy.Downregulation of SNAI1 enhances the sensitivity of ESCA cells to ionizing radiation(IR),resulting in remarkable tumor regression upon IR treatment in vivo.This study underscores the direct involvement of SNAI1 in the regulation of EMT,particularly under IR-induced conditions.Furthermore,inhibiting deacetylation effectively suppresses EMT,suggesting a potential avenue to enhance the response to radiotherapy in ESCA.CONCLUSION This study highlights SNAI1's role in ESCA radiosensitivity,offering prognostic insights and therapeutic strategies to enhance radiotherapy by targeting SNAI1 and modulating EMT processes.

关 键 词：Esophageal cancer RADIOSENSITIVITY Snail family transcriptional repressor 1 BIOINFORMATICS Epithelial-mesenchymal transition 

分 类 号：R73[医药卫生—肿瘤]