20-Hydroxyecdysone Partially Alleviates Ischemia/Reperfusion-Induced Damage of Mouse Hind Limb Skeletal Muscle  

作　　者：Alena A.Semenova Anastasia D.Igoshkina Alena A.Cherepanova Natalia V.Mikina Anastasia E.Stepanova Olga E.Krasnoshchekova Vyacheslav A.Sharapov Rimma G.Savchenko Lyudmila V.Parfenova Mikhail V.Dubinin 

机构地区：[1]Department of Biochemistry,Cell Biology,and Microbiology,Mari State University,Yoshkar-Ola,424001,Russia [2]Institute of Petrochemistry and Catalysis,Russian Academy of Sciences,Ufa,450075,Russia

出　　处：《BIOCELL》2025年第3期437-450,共14页生物细胞(英文)

基　　金：supported by a grant from the Russian Science Foundation(23-75-01061)。

摘　　要：Objectives:Skeletal muscle ischemia/reperfusion injury(IRI)occurs as a result of a marked reduction in arterial perfusion to a limb and can lead to tissue death and threaten limb viability.This work assessed the effects of 20-hydroxyecdysone(20E)on hindlimb skeletal tissue following tourniquet-induced ischemia/reperfusion injury.Methods:Animals were divided into 4 groups—control group(Control),Control+20E(C+20E),mice with IRI(IRI),and mice with IRI+20E(IRI+20E).IRI was modeled by applying a tourniquet to the hind limb for 2 h with reperfusion for 1 h.5 mg/kg of 20E was administered intraperitoneally for 14 days.Afterward,the physical activity of mice,the histological structure of the quadriceps femoris,the expression of genes encoding proteins induced by hypoxia and involved in tissue adaptation to ischemia,and the functional parameters of skeletal muscle mitochondria were assessed.Results:It was shown that IRI of the limbs leads to functional disorders,depression of muscle function,accumulation of malondialdehyde(MDA)in mitochondria,and a decrease in their Ca2+buffering capacity,as well as an increase in the expression of HIF-1α,VGEF-A,PGC1αand PDGF-BB genes associated with adaptation to ischemia.20E reduced the intensity of degenerative processes in skeletal muscles,which was expressed in a decrease in the number of centrally nucleated fibers.Analysis of gene expression levels indicated a high degree of adaptation of animals to IRI.20E reduced the level of MDA in mitochondria,but did not affect the rate of respiration and calcium retention capacity of organelles both in normal conditions and during IRI.Conclusion:20E partially alleviates the skeletal muscle damage caused by IRI and can be used as part of combination therapy.

关 键 词：Skeletal muscle ISCHEMIA/REPERFUSION 20-HYDROXYECDYSONE MITOCHONDRIA oxidative stress 

分 类 号：R73[医药卫生—肿瘤]