辣木固体饮料的开发及其对小鼠的减肥作用研究  

Development of Moringa oleifera Powdered Beverage and its Effects on Weight Loss

作　　者：吴宽 赵存朝 田洋[1,3] 鲁涛 WU Kuan;ZHAO Cunchao;TIAN Yang;LU Tao(College of Food Science and Technology,Yunnan Agricultural University,Kunming,Yunnan 650201,China;Yunnan Plateau Characteristic Agricultural Industry Research Institute,Kunming,Yunnan 650201,China;Yunnan Province Engineering Research Center for Food and Medicine with the Same Origin and Functional Foods,,Kunming,Yunnan 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]云南省高原特色农业产业研究院,云南昆明650201 [3]云南省药食同源功能食品工程研究中心,云南昆明650201

出　　处：《热带农业科学》2025年第2期110-116,共7页Chinese Journal of Tropical Agriculture

基　　金：重要药食兼用资源生物制造技术开发与应用(No.202002AA100005);国家木薯产业技术体系(辣木产品加工岗位)(No.CARS-11-YNTY)。

摘　　要：以辣木叶发酵提取物为主要原料,通过与蓝莓果粉、麦芽糊精、赤藓糖醇、柠檬酸等辅料调配,研制得一款辣木固体饮料,并探究其对小鼠减肥的作用。在单因素实验基础上,利用响应面优化辣木固体饮料的配方;选取7周龄SPF级雄性C57BL/6小鼠40只,将其随机分为5组,正常对照组、模型对照组、辣木固体饮料低剂量组(100 mg/kg·BW)、中剂量组(200 mg/kg·BW)、高剂量组(400 mg/kg·BW),灌胃体积0.1 mL/10g·BW,正常饲料喂养正常对照组小鼠,其余各组给予高脂饲料,干预56d。实验期间,记录小鼠的体重变化;实验结束后,处死小鼠,取皮下、附睾和肾周围白色脂肪组织,计算脂肪系数。结果表明:当麦芽糊精添加量为18 g、辣木发酵提取物与蓝莓果粉质量比为1∶1、赤藓糖醇添加量为20 g时,得到的辣木固体饮料口感最好,感官评分最高,且理化性质和微生物指标均符合国家标准,长期稳定性良好。动物实验结果表明,与模型对照组相比,辣木固体饮料中剂量组小鼠体重、脂肪湿重和脂肪系数显著降低(p<0.05),高剂量组小鼠体重、脂肪湿重和脂肪系数极显著降低(p<0.01),说明辣木固体饮料具有减少小鼠体内脂肪堆积、降低脂肪含量的作用。In this study,the fermented extract of Moringa oleifera leaves was used as the primary raw material.By mixing with blueberry fruit powder,maltodextrin,erythritol,citric acid,and other auxiliary materials,an M.oleifera powdered beverage with a weight loss effect was developed,and its effect on weight loss in mice was explored.Based on single-factor experiments,the formulation of M.oleifera powdered beverages was optimized using response surface methodology.Forty SPF male C57BL·6 mice–1 aged seven weeks were randomly divided into five groups:the regular control group,model control group,M.oleifera powdered beverage low-dose(100 mg·kg^(–1)BW),medium-dose(200 mg·kg^(–1)BW)and high-dose(400 mg·kg^(–1)BW)groups.The intragastric volume was 0.1 mL·10 g^(–1)BW.The regular control group was fed a normal diet,while the other groups were fed a high-fat diet for 56 days.During the experiment,the weight changes of the mice were recorded.After the experiment,the mice were sacrificed,and subcutaneous,epididymal,and perirenal white adipose tissue were collected to calculate the fat coefficient.The results revealed that when the maltodextrin dosage was 18 g,the ratio of M.oleifera fermented extract to blueberry fruit powder was 1:1.The erythritol dosage was 20 g,the M.oleifera powdered beverage had the best taste,the highest sensory score,and its physicochemical properties and microbial indices met national standards with good long-term stability.The animal experiment results showed that compared with the model control group,the body weights,wet fat weights,and fat coefficients of the mice in the medium and high-dose groups of M.oleifera powdered beverage were significantly lower(p<0.05 or p<0.01),indicating that M.oleifera powder beverage can reduce the accumulation of fat in the bodies of the mice and lower fat content.

关 键 词：辣木叶 发酵 固体饮料 减肥 

分 类 号：TS201.4[轻工技术与工程—食品科学]