降糖活性乳酸菌的筛选及对2型糖尿病小鼠的改善作用  

Screening and Evaluation of Lactic Acid Bacteria for Hypoglycemic Effect in Type 2 Diabetic Mellitus Mice

作　　者：杨晓华 洪嘉淇 王临好 潘丽怡 郭何子贤 王洁 廖振林[1] YANG Xiaohua;HONG Jiaqi;WANG Linhao;PAN Liyi;GUO Hezixian;WANG Jie;LIAO Zhenlin(College of Food Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《食品科学》2025年第8期151-161,共11页Food Science

基　　金：广东省省级科技计划项目(2024B0202010001)。

摘　　要：通过甘蔗渣自然发酵筛选出一株具有高效降糖效果的益生菌。通过体外抑制关键消化酶和体内高脂饮食联合链脲佐菌素诱导的2型糖尿病小鼠模型评估菌株及其发酵上清液的降糖效果。结果表明,从发酵甘蔗渣中共筛选得到6株植物乳植杆菌(Lactiplantibacillus plantarum),其中植物乳植杆菌P6发酵上清液对α-葡萄糖苷酶的抑制率达到96.49%;动物体内实验表明,植物乳植杆菌P6及其发酵上清液能有效改善小鼠多饮、多食、多尿以及体质量减轻、脏器肿大等症状,并显著改善糖耐量和胰岛素分泌异常;此外,能有效降低血清中甘油三酯、总胆固醇、低密度脂蛋白、炎症因子(白细胞介素-6、白细胞介素-10、肿瘤坏死因子-α和脂多糖)的含量,并促进高密度脂蛋白、胰高血糖素样肽-1的分泌。因此,植物乳植杆菌P6及其发酵上清液具有显著的降糖效果和改善糖尿病相关症状的潜力。This study isolated and identified a probiotic strain with hypoglycemic activity from naturally fermented sugarcane residue.The inhibitory effects of the strain and its cell-free supernatant against key digestive enzymes were assessed along with hypoglycemic effects in a mouse model of type 2 diabetes mellitus induced by a high-fat diet and streptozotocin.The results showed that six strains of Lactiplantibacillus plantarum were obtained from naturally fermented sugarcane residue.Among them,the cell-free supernatant of L.plantarum P6 inhibited 96.49%ofα-glucosidase activity.The in vivo experiment showed that L.plantarum P6 and its cell-free supernatant could effectively alleviate symptoms such as excessive drinking,eating,and urination,as well as body mass loss and organ enlargement in mice.It could also significantly rescue glucose tolerance and abnormal insulin secretion,effectively reduce the serum levels of triglycerides,total cholesterol,low-density lipoprotein cholesterol,and inflammatory factors(interleukin(IL)-6,IL-10,tumor necrosis factor-α,and lipopolysaccharide),and promote the secretion of high-density lipoprotein cholesterol and glucagon-like peptide-1.Therefore,L.plantarum P6 and its cell-free supernatant have the potential to significantly reduce blood sugar levels and mitigate diabetes-related symptoms.

关 键 词：植物乳植杆菌 发酵上清液 Α-葡萄糖苷酶 降糖能力 2型糖尿病 

分 类 号：TS201.4[轻工技术与工程—食品科学]