4-甲氧基肉桂醛对柑橘果实采后青霉病抗性的影响  

Effect of 4-Methoxycinnamaldehyde on Postharvest Resistance to Blue Mold in Citrus Fruit

作　　者：黄露露 侯天玉 张欣雨 吴仪青 陈楚英[1] 陈金印[1] HUANG Lulu;HOU Tianyu;ZHANG Xinyu;WU Yiqing;CHEN Chuying;CHEN Jinyin(Jiangxi Provincial Key Laboratory for Postharvest Storage and Preservation of Fruits&Vegetables,School of Agricultural Sciences,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学农学院,果蔬贮藏与保鲜江西省重点实验室,江西南昌330045

出　　处：《食品科学》2025年第8期302-310,共9页Food Science

基　　金：国家自然科学基金地区科学基金项目(32060703);赣鄱俊才支持计划·青年科技人才托举项目(2023QT02);江西省农业关键核心技术攻关项目(JXNK202304-04)。

摘　　要：为明确4-甲氧基肉桂醛(4-methoxycinnamaldehyde,4-MCA)对柑橘采后青霉病的防控,以纽荷尔脐橙果实为研究对象,采用无菌水为对照组,1000 mg/L 4-MCA溶液为处理组,在(27±1.0)℃、90%~95%相对湿度的条件下连续培养4 d,每24 h取样测定果实在接种意大利青霉(Penicillium italicum)后的病斑直径、病情指数、pH值、可滴定酸含量、有机酸组分含量及相关酶活性和基因表达量的变化。结果表明,4-MCA处理有效延缓了脐橙果实在P.italicum侵染过程中可滴定酸含量的上升和pH值的下降,显著降低了病斑直径和病情指数,抑制了青霉病的发生几率。同时,4-MCA处理抑制了磷酸烯醇式丙酮酸羧化酶、苹果酸脱氢酶、苹果酸酶、柠檬酸合成酶活性的增加及相关编码基因的上调表达,还提高了顺乌头酸酶和异柠檬酸脱氢酶活性并上调其基因表达,抑制脐橙果皮中有机酸的积累。以上研究结果说明4-MCA处理能通过调控有机酸代谢增强脐橙果实对采后青霉病的抗性,为柑橘果实的真菌病害防控提供新的见解和理论依据。In order to explore the inhibitory effect of 4-methoxycinnamaldehyde(4-MCA)treatment on postharvest blue mold and organic acid metabolism in citrus fruit,‘Newhall’navel oranges were soaked in 1000 mg/L 4-MCA or distilled water as a control,artificially wounded,inoculated with Penicillium italicum and stored at(27±1.0)℃and relative humidity of 90%–95%for up to 96 h.Lesion diameter(LD),disease index(DI),pH,titrable acid content,organic acid contents,and the activity and gene expression of organic acid-related enzymes were measured every 24 h.Results indicated that 4-MCA treatment effectively delayed the decline in pH and the increase in titratable acid content during the whole period of P.italicum infection,significantly reduced LD and DI,and inhibited the incidence of blue mold.Compared with the control group,4-MCA treatment inhibited the increase in the activities and gene expression of phosphoenolpyruvate carboxylase,malate dehydrogenase,malic enzyme,and citrate synthase.Meanwhile,4-MCA treatment increased the activities and gene expression of cis-aconitase and isocitrate dehydrogenase,thereby inhibiting the accumulation of organic acids in orange peels.The above results indicate that 4-MCA treatment can enhance the resistance to P.italicum infection in harvested‘Newhall’navel oranges by regulating organic acid metabolism,providing new insights and a theoretical basis for the prevention and management of fungal diseases in citrus fruit.

关 键 词：脐橙果实 4-甲氧基肉桂醛 意大利青霉 柑橘青霉病 有机酸代谢 

分 类 号：S436.661[农业科学—农业昆虫与害虫防治]