关于第几轮PCR获得等长的DNA的思考及教学建议  

In which round of PCR cycle does equimolar DNA fragments appear:Thought and teaching suggestions

作　　者：张小春[1] 吴久宏[2] 冯骞 ZHANG Xiaochun;WU Jiuhong;FENG Qian(Jiangsu Haian Senior High School,Nantong 226600,China;Jiangsu Lishui Senior High School,Nanjing 211200,China;School of Life Sciences,Tsinghua University,Beijing 100084,China)

机构地区：[1]江苏省海安高级中学,南通226600 [2]江苏省溧水高级中学,南京211200 [3]清华大学生命科学学院,北京100084

出　　处：《生物学教学》2025年第4期86-89,共4页Biology Teaching

基　　金：江苏省教育科学“十四五”规划“苏教名家”专项课题“指向深度学习的生物学大单元教学设计的实践研究”,No.SJMJ/2023/17。

摘　　要：自2011年江苏高考题考查过PCR第3轮循环产物中开始出现等长的DNA片段后,关于等长DNA片段的考查总是伴随着争论与疑惑,本文对此问题进行分析,并给出相应的教学建议。Since a test question on equimolar DNA fragments in the products of the third round of PCR cycle appeared in the 2011 Jiangsu college entrance exam,arguments and doubts about test questions on equimolar DNA fragments have persisted.This paper analyzes this issue and provides corresponding teaching suggestions.

关 键 词：PCR 等长DNA 教学建议 

分 类 号：G633.91[文化科学—教育学]