产细菌素乳酸菌Lpe59的选育及其细菌素基因簇的挖掘  

Screening of bacteriocin-producing lactic acid bacteria Lpe59 andmining of bacteriocin gene cluster

作　　者：张宗源 岳丹丹[1] 周留柱 王雪妍[1] 陈登辉 潘梦诗 徐宏光 李冠杰[1] 尹文兵[2] ZHANG Zongyuan;YUE Dandan;ZHOU Liuzhu;WANG Xueyan;CHEN Denghui;PAN Mengshi;XU Hongguang;LI Guanjie;YIN Wenbing(Henan Academy of Science Institute of Biology Co.,Ltd.,Zhengzhou 450008,China;State Key Laboratory of Myology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]河南省科学院生物研究所有限责任公司,郑州450008 [2]中国科学院微生物研究所真菌学国家重点实验室,北京100101

出　　处：《生物学杂志》2025年第2期33-39,共7页Journal of Biology

基　　金：河南省科技攻关项目(232102110100);河南省科学院杰出青年人才培养项目(210405010);河南省科学院基本科研业务费项目(240605014);河南省科学院省级特聘研究员项目(230505002)。

摘　　要：为了筛选一株产细菌素乳酸菌,并对其进行细菌素基因簇的挖掘,以大肠杆菌、金黄色葡萄球菌为指示菌,通过常规方法初筛和复筛后筛选出1株抑菌能力较强的产细菌素的戊糖乳杆菌Lpe59。全基因组测序结果显示,菌株Lpe59基因组大小为3274558 bp,编码3047个基因。在COG、GO、KEGG、CAZy和VFDB数据库中分别注释到2434、2203、1614、107和4个功能基因。应用在线软件AntiSMASH 6.11预测到该菌具有核糖体合成和翻译后修饰肽类(RiPP-like)、Ⅲ型聚酮(T3PKS)化合物、萜烯(terpene)和cyclic-lactone-autoinducer等4种次级代谢物基因,通过Bagel4预测到一条合成Ⅱ类细菌素Plantaricin K和细菌素Plantaricin EF基因簇。通过抑菌试验和基因组预测表明戊糖乳杆菌Lpe59是一株极具生防潜力的产细菌素乳酸菌,研究结果为其细菌素Plantaricin K和Plantaricin EF进一步开发应用提供理论基础。In order to screen a bacteriocin-producing lactic acid bacteria and explore its bacteriocin gene cluster,the pathogens Escherichia coli and Staphylococcus aureus were used as indicator,through initial and repeated screenings using conventional methods,Lactobacillus pentosus Lpe59,a strain with strong antibacterial activity and bacteriocin production was isolated.The whole-genome information of strain Lpe59 was obtainde through high-throughput sequencing,the results showed the genome size of strain Lpe59 was 3274558 bp,encoding 3047 genes,with 2434,2203,1614,107 and 4 functional genes annotated in COG,GO,KEGG,CAZY and VFDB databases,respectively.Using the online software AntiSMASH 6.11,four types of secondary metabolite gene clusters were predicted in strain Lpe59,including ribosomally synthesized and post-translationally modified peptide-like(RiPP-like)compounds,typeⅢpolyketide synthase(T3PKS)metabolites,terpene,and cyclic-lactone-autoinducer.Additionally,a gene cluster for the synthesis of classⅡbacteriocins Plantaricin K and Plantaricin EF was predicted through Bagel4.In conclusion,antibacterial tests and genomic predictions suggested that L.pentosus Lpe59 was a promising lactic acid bacterium with biocontrol potential and the ability to produce bacteriocins.The research findings provided a theoretical basis for the further development and application of its bacteriocins,Plantaricin K and Plantaricin EF.

关 键 词：戊糖乳杆菌 细菌素 抑菌 全基因组 合成基因簇 

分 类 号：TS201.3[轻工技术与工程—食品科学]