基于加权基因共表达网络分析探讨急性胰腺炎相关脓毒症潜在的关键基因  

Identification of key genes participating in the progression of acute pancreatitis-related sepsis

作　　者：桑珍珍[1] 冯顺易[1] 李勇[1] SANG Zhenzhen;FENG Shunyi;LI Yong(Department of Emergency Medicine,Cangzhou Central Hospital,Cangzhou,Hebei 061001,China)

机构地区：[1]沧州市中心医院急诊科,河北沧州061001

出　　处：《安徽医药》2025年第5期940-944,I0002,I0003,I0004,共8页Anhui Medical and Pharmaceutical Journal

基　　金：河北省科技厅医学科学研究重点计划项目(182777156)。

摘　　要：目的旨在识别急性胰腺炎相关脓毒症的关键基因。方法2023年3―9月从GEO数据库下载基因数据集。采用WGCNA确定急性胰腺炎和脓毒症的枢纽模块,并将两个模块交叉以确定与急性胰腺炎相关脓毒症有关的共同基因。采用R软件筛选出急性胰腺炎和脓毒症的差异表达基因,同时结合WGCNA和差异分析的结果,得到与急性胰腺炎和脓毒症相关的候选基因,最后将两者取交集得到急性胰腺炎相关脓毒症的关键基因。利用GO和KEGG对急性胰腺炎和脓毒症的交集差异表达基因进行功能富集分析。结果WGCNA分析得到6个急性胰腺炎相关枢纽模块和12个脓毒症相关枢纽模块,将其交叉后共获得161个共同基因。结合WGCNA和差异分析的结果,得出与急性胰腺炎和脓毒症相关的候选基因,通过将候选基因交叉,共筛选出11个关键基因(CRISP3、ENTPD7、ERLIN1、HK3、JAK2、KLRF1、MMP9、NEU1、PLP2、SH3GLB1、TP53I3)。根据功能富集分析,急性胰腺炎相关脓毒症的关键基因主要在免疫和炎症相关通路中增强。结论CRISP3、ENTPD7、ERLIN1、HK3、JAK2、KLRF1、MMP9、NEU1、PLP2、SH3GLB1、TP53I3可能是参与急性胰腺炎相关脓毒症的关键基因。Objective To identify key genes involved in acute pancreatitis associated sepsis.Methods Gene datasets were downloaded from GEO database between March and September 2023.WGCNA was used to identify the pivotal modules of acute pancreatitis and sepsis,and the two modules were crossed to identify common genes associated with acute pancreatitis associated sepsis.Differentially expressed genes of acute pancreatitis and sepsis were screened using R software.Combined with the results of WGCNA and differential analysis,candidate genes associated with acute pancreatitis and sepsis were obtained.Finally,the key genes of acute pancreatitis associated sepsis were obtained by intersection of the two.Functional enrichment analysis of the intersecting differentially expressed genes between acute pancreatitis and sepsis was conducted using GO and KEGG.Results Through WGCNA analysis,six hub modules associated with acute pancreatitis and twelve hub modules related to sepsis were identified.The intersection of these modules yielded a total of 161 common genes.Combining the results of WGCNA and difference analysis,candidate genes associated with acute pancreatitis and sepsis were obtained.By crossing candidate genes,A total of 11 key genes(CRISP3,ENTPD7,ERLIN1,HK3,JAK2,KLRF1,MMP9,NEU1,PLP2,SH3GLB1,TP53I3)were screened.According to functional enrichment analysis,the key genes in acute pancreatitis associated sepsis were mainly enhanced in immune and inflammation-related pathways.Conclusion CRISP3,ENTPD7,ERLIN1,HK3,JAK2,KLRF1,MMP9,NEU1,PLP2,SH3GLB1,TP53I3 may be the key genes involved in acute pancreatitis associated sepsis.

关 键 词：急性胰腺炎 脓毒症 关键基因 加权基因共表达网络分析 基因本体论 

分 类 号：R576[医药卫生—消化系统]