黄酒循环浸米中核心微生物群落组装  

Assembly of core microbial community in cyclic rice soaking of Huangjiu

作　　者：叶书建 刘甜甜 毛健[1,2,3] 刘双平 YE Shujian;LIU Tiantian;MAO Jian;LIU Shuangping(National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Jiangnan University(Shaoxing)Industrial Technology Research Institute,Key Laboratory of Traditional Fermentation Food and Health,Shaoxing 312000,China;National Engineering Research Center for Huangjiu,Shaoxing 312000,China)

机构地区：[1]江南大学食品学院,粮食发酵与食品生物制造国家工程研究中心,江苏无锡214122 [2]江南大学(绍兴)产业技术研究院,传统酿造食品健康功效重点实验室,浙江绍兴312000 [3]国家黄酒工程技术研究中心,浙江绍兴312000

出　　处：《食品与发酵工业》2025年第8期77-84,共8页Food and Fermentation Industries

基　　金：国家重点研发计划课题(2022YFD2101204);绍兴科技计划项目(2022B43001);绍兴科技计划项目(2023B43001)。

摘　　要：循环浸米工艺被誉为“绿色技术”,但复杂的工厂微生态环境阻碍了它的工业化应用。为了抵御循环浸米过程中的产胺微生物入侵,实现生物胺“零增长”及多批次循环。该研究首先发现循环浸米过程中生物胺代谢是微生物交互作用的结果。通过相关性分析发现,植物乳植物杆菌(Lactiplantibacillus plantarum)、有害片球菌(Pediococcus damnosus)、弯曲广泛乳杆菌(Latilactobacillus curvatus)、棒状腐败乳杆菌(Loigolactobacillus coryniformis)、短促生乳杆菌(Levilactobacillus brevis)与米浆水中生物胺的降低显著相关;基于预测结果,对米浆水中核心微生物群落进行了筛选和构建,验证实验中生物胺抑制效果达到了55.21%;最后开发了一种合成微生物组循环浸米工艺,在模拟工厂循环浸米实验中实现了生物胺的“零增长”。结果表明,构建的L.plantaurm、L.brevis、L.curvatus群落具有强抗产胺微生物入侵的能力,为循环浸米的工业化应用创造了可行性。The cyclic soaking process,hailed as a“green technology”,faces challenges in industrial application due to the complex microbial ecology within factory environments.To combat the intrusion of biogenic amines-producing microorganisms during the cyclic soaking process,achieving“zero growth”of biogenic amines and multi-batch cycling,this study first elucidated that the metabolism of biogenic amines during cyclic soaking was a result of microbial interactions.Correlation analysis revealed significant associations between Lactiplantibacillus plantarum,Pediococcus damnosus,Latilactobacillus curvatus,Loigolactobacillus coryniformis,Levilactobacillus brevis,and the reduction of biogenic amines in rice seriflux.Based on predictive results,the core microbial community in rice seriflux was selected and constructed,demonstrating a 55.21%inhibition of biogenic amines in verification experiments.Finally,a synthetic microbial consortium-based cyclic soaking process was developed,achieving“zero growth”of biogenic amines in simulated factory cyclic soaking process experiments.Experimental results indicated that the constructed communities of L.plantaurm,L.brevis,and L.curvatus exhibited strong resistance to intrusion by biogenic amines-producing microorganisms,thereby creating feasibility for the industrial application of the cyclic soaking process.

关 键 词：生物胺 微生物群落构建 黄酒 循环浸米 

分 类 号：TS261.1[轻工技术与工程—发酵工程]