生成和处理条件对等离子体活化海水杀灭腐败希瓦氏菌(Shewanella putrefaciens)效果的影响  

Effects of generation and treatment conditions on plasma-activated seawater on inactivation of Shewanella putrefaciens

作　　者：彭行健 贾世亮 刘书来[1,2,3] 周绪霞 丁玉庭[1,2,3] 柯志刚 PENG Xingjian;JIA Shiliang;LIU Shulai;ZHOU Xuxia;DING Yuting;KE Zhigang(College of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China;Zhejiang Key Laboratory of Green,Low-carbon and Efficient Development of Marine Fishery Resources,Hangzhou 310014,China;National R&D Branch Center for Pelagic Aquatic Products Processing(Hangzhou),Hangzhou 310014,China)

机构地区：[1]浙江工业大学食品科学与工程学院,浙江杭州310014 [2]浙江省全省深蓝渔业资源绿色低碳高效开发重点实验室,浙江杭州310014 [3]国家远洋水产品加工技术研发分中心(杭州),浙江杭州310014

出　　处：《食品与发酵工业》2025年第8期151-156,共6页Food and Fermentation Industries

基　　金：浙江省“领雁”研发攻关计划项目(2022C02025);浙江省自然科学基金项目(LY24C200006)。

摘　　要：该文研究了一种新型海产品船载保鲜方法,即利用非热等离子体(non-thermal plasma,NTP)处理海水,制得等离子体活化海水(plasma-activated seawater,PASW)。实验选用盐含量为35 g/L的海水进行电晕放电处理制备PASW,以海产品中常见的腐败菌——腐败希瓦氏菌(Shewanella putrefaciens)为研究对象,探讨了PASW的生成和处理条件对其杀菌能力的影响。研究发现,气体组成、NTP电压、NTP频率、放电距离等生成条件和反应温度、贮藏时间等处理条件对PASW杀灭S.putrefaciens的效果产生显著影响,而贮藏温度对其杀菌能力的影响不显著。在以空气为工作气体、峰值电压为25 kV、频率为10 kHz、放电距离为10 mm、放电300 s条件下制备的PASW,其在25℃条件下可杀灭(5.12±0.14)lg CFU/mL的S.putrefaciens。研究结果可为推广PASW在海产品减菌保鲜领域的应用提供重要的基础数据和科学依据。This study introduced an innovative method for preserving seafood onboard by utilizing non-thermal plasma(NTP)to treat seawater,resulting in the creation of plasma-activated seawater(PASW).Experiments were carried out using seawater with a salinity of 35 g/L to generate PASW through corona discharge.Shewanella putrefacien,a common bacterium responsible for seafood spoilage,was chosen as the focal point of the study to investigate the impact of PASW generation and treatment conditions on its antibacterial properties.The study revealed that various factors such as gas composition,NTP voltage,NTP frequency,discharge distance,as well as reaction temperature and storage duration significantly influenced the efficacy of PASW in inactivating S.putrefaciens.However,storage temperature was found to have no significant effect on its antibacterial effectiveness.PASW produced under specific conditions involving the use of air as the working gas,a peak voltage of 25 kV,a frequency of 10 kHz,a discharge distance of 10 mm,and a discharge duration of 300 seconds,was able to deactivate(5.12±0.14)lg CFU/mL of S.putrefaciens at 25℃.The outcomes of this study offer crucial foundational information and a scientific basis for the application of PASW in the realm of seafood preservation.

关 键 词：等离子体活化海水 腐败希瓦氏菌 生成条件 处理条件 减菌效果 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]