植物乳植杆菌-30产胞外多糖的结构表征及降尿酸作用  

Structural Characterization and Anti-hyperuricemic Activity of Exopolysaccharides Secreted by Lactiplantibacillus plantarum-30

作　　者：韩冬 张佳辉 江正强[1] 常畅 HAN Dong;ZHANG Jiahui;JIANG Zhengqiang;CHANG Chang(Key Laboratory of Food Bioengineering(China National Light Industry),College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,中国轻工业食品生物工程重点实验室,北京100083

出　　处：《食品科学》2025年第9期148-155,共8页Food Science

基　　金：“十四五”国家重点研发计划重点专项(2022YFD2101403)。

摘　　要：本研究筛选得到1株高产胞外多糖(exopolysaccharide,EPS)的菌株,确定其为植物乳植杆菌(Lactiplantibacillus plantarum),命名为HZB-30,并对其生化性质等进行分析。结果表明,该菌株在发酵过程中产酸能力显著,发酵28 h后EPS产量达到230.66 mg/L。HZB-30产EPS分子质量为14639 Da,含有葡萄糖(37.76%)、半乳糖(19.15%)等多种单糖组分,具有较高的黄嘌呤氧化酶(xanthine oxidase,XO)抑制率(80.2%)。经体外模拟消化后,HZB-30产EPS的消化率为20.7%,且仍具有XO抑制活性(43.3%)。通过构建高尿酸HK-2细胞模型发现,HZB-30产EPS(20~60μg/mL)能够有效降低细胞中肿瘤坏死因子-α、白细胞介素(interleukin,IL)-1β、IL-6和IL-8的水平,减少尿酸生成。本实验结果可为HZB-30产EPS在降尿酸功能食品中的应用奠定理论和实践基础。This study obtained a strain producing high yield of exopolysaccharide(EPS),which was identified as Lactiplantibacillus plantarum HZB-30.The strain produced 230.66 mg/L of EPS after fermentation for 28 h.The molecular mass of EPS produced by HZB-30 was 14639 Da,which was composed of several monosaccharides,such as glucose(37.76%)and galactose(19.15%).The EPS had high inhibitory activity against xanthine oxidase(XO)with an inhibition rate of 80.2%.After in vitro simulated digestion,the digestibility of the EPS was 20.7%,and 43.3%of the initial xanthine oxidase inhibitory activity remained.In an HK-2 cell model of hyperuricemia,the EPS at 20–60μg/mL effectively decreased the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,and IL-8 and inhibited the accumulation of uric acid.The findings from this study are both theoretically and practically important for the application of HZB-30-derived EPS to develop anti-hyperuricemic food products.

关 键 词：植物乳植杆菌 胞外多糖 结构表征 降尿酸作用 

分 类 号：TS202.1[轻工技术与工程—食品科学] Q939.97[轻工技术与工程—食品科学与工程]