副地衣芽孢杆菌Q13缓解氧嗪酸钾诱导的小鼠高尿酸血症作用  

Effect of Bacillus paralicheniformis Q13 on Alleviating Hyperuricemia Induced by Potassium Oxonate in Mice

作　　者：索铃兰 李月 姜斌[1] 冷玥 任大勇[1] SUO Linglan;LI Yue;JIANG Bin;LENG Yue;REN Dayong(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《食品科学》2025年第9期177-188,共12页Food Science

基　　金：吉林省科技厅中青年科技创新创业卓越人才(团队)项目(20220508106RC)。

摘　　要：目的:寻找一种新的能够缓解高尿酸血症(hyperuricemia,HUA)的益生菌菌株,并分析其对HUA的抑制效果。方法:所有小鼠随机分成4组(空白对照组:CON;HUA模型组:HUA;副地衣芽孢杆菌Q13干预组:Q13;别嘌呤醇干预组:ADC),每组6只小鼠,连续造模及给药,共持续14 d。采集血液、肾脏、肝脏、结肠和粪便,用于后续分析。采用纸片覆盖扩散法评估13种抗生素的敏感性,并评估其对胃液和肠液的耐受性。结果:Q13能有效缓解小鼠血清的尿酸水平升高,抑制肝脏中黄嘌呤氧化酶的活性(P<0.01),并保护肾功能。此外,Q13提高了小鼠肝脏超氧化物歧化酶活性(P<0.01),降低了肝脏丙二醛水平(P<0.01);有助于维持正常高密度脂蛋白胆固醇水平,能够显著降低低密度脂蛋白胆固醇水平(P<0.05);降低肝脏内毒素水平(P<0.01),抑制肾脏白细胞介素-1β和肿瘤坏死因子-α炎症因子的分泌(P<0.01),改善肾脏、肝脏和结肠的组织病理状态;改善氧嗪酸钾诱导HUA所致肠道菌群失衡,使拟杆菌门与厚壁菌门的比例(Bac/Firm比值)恢复到CON组的水平,降低了与炎症相关的TM7菌门丰度。Q13对13种抗生素均具有敏感性或中度敏感。Q13在4 h内人工胃液存活率为95%以上,人工肠液存活率为86%以上。结论:副地衣芽孢杆菌Q13表现出优秀的抗尿酸升高能力,能够改善肾脏功能,显著下调黄嘌呤氧化酶活性,抑制氧嗪酸钾诱导的小鼠肾脏、肝脏和结肠组织损伤,减轻肾脏炎症状态,提高肝脏抗氧化活性,调节肠道菌群稳态,最终达到控制HUA的作用。本研究可为Q13开发为预防和改善HUA的益生菌及相关产品提供理论支撑。Objective:To find a novel probiotic strain capable of effectively alleviating hyperuricemia(HUA)and to validate its inhibitory effect on HUA in an animal model.Methods:All mice were randomly divided into four groups(n=6 per group):control(CON),HUA model,Bacillus paralicheniformis Q13 intervention(Q13),and allopurinol intervention(ADC).The modeling and administration lasted for a total of 14 days.One hour after the final administration,blood,kidney,liver,colon,and feces samples were collected from each group for subsequent analysis.The disc diffusion method was employed to evaluate the susceptibility of this strain to 13 antibiotics and the tolerance to gastric and intestinal fluids was assessed.Results:Q13 effectively alleviated the elevation of serum uric acid levels,inhibited the activity of xanthine oxidase in the liver(P<0.01),and protected renal function in mice.Furthermore,Q13 significantly increased hepatic superoxide dismutase(SOD)activity(P<0.01)and decreased hepatic malondialdehyde(MDA)content(P<0.01)in mice.It helped maintain normal high-density lipoprotein cholesterol(HDL-C)levels while significantly reducing low-density lipoprotein cholesterol(LDL-C)levels(P<0.05).Q13 also reduced endotoxin levels in the liver(P<0.01)and inhibited the secretion of renal inflammatory cytokines such as interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)(P<0.01),as well as ameliorating the histopathological changes of the kidney,liver,and colon.Moreover,it rectified the dysbiosis of gut microbiota induced by potassium oxonate, restoring the Bacteroidetes-to-Firmicutes ratio (Bac/Firm ratio) to the level of theCON group and decreasing the abundance of the inflammation-associated phylum TM7. Q13 was susceptible or moderatelysusceptible to all 13 antibiotics. The 4-h survival rate of Q13 was over 95% in artificial gastric juice and over 86% in artificialintestinal juice. Conclusion: B. paralicheniformis Q13 significantly inhibited the elevation of uric acid and enhanced renalfunction. Moreover, it significantly dow

关 键 词：副地衣芽孢杆菌 高尿酸血症 肾损伤 黄嘌呤氧化酶 氧化应激 

分 类 号：TS201.3[轻工技术与工程—食品科学] R589.7[轻工技术与工程—食品科学与工程]