副干酪乳杆菌Lp.R3的高密度培养工艺优化  

Optimization of High-density Culture Process of Lacticaseibacillus paracasei Lp.R3

作　　者：方诗会 熊尧 张召 林俊芳[1] 陈涛 郭丽琼[1] FANG Shihui;XIONG Yao;ZHANG Zhao;LIN Junfang;CHEN Tao;GUO Liqiong(Department of Bioengineering,College of Food Science,South China Agricultural University,Guangzhou 510640,China;Guangdong Longsee Biomedical Corporation,Guangzhou 510535,China)

机构地区：[1]华南农业大学食品学院生物工程系,广东广州510640 [2]广东南芯医疗科技有限公司,广东广州510535

出　　处：《食品工业科技》2025年第9期196-205,共10页Science and Technology of Food Industry

基　　金：广东省重点领域研发计划项目(2022B0202050002);国家自然科学基金项目(32072646,32272785);2021年度佛山市科技创新团队(青年拔尖人才)(2120001010795)。

摘　　要：副干酪乳杆菌Lp.R3(Lacticaseibacillus paracasei Lp.R3)是一株具有优良功能的益生菌,为实现其产业化应用对其进行高密度培养以提高活菌数。本研究通过单因素实验和正交试验优化,考察了培养基成分、静态培养条件以及发酵罐工艺对Lp.R3活菌数的影响。确定了优化后的最佳培养基配方为葡萄糖30 g/L、酵母浸粉50 g/L、无水乙酸钠10 g/L、硫酸锰0.1 g/L、吐温801 g/L;最佳培养条件为温度35℃、初始pH7、接种量为1%,此时活菌数为4.75×10^(9)CFU/mL,是MRS培养基的2.91倍;在5 L发酵罐中的最佳发酵工艺为:不通气搅拌、发酵过程恒定pH为5、25%的氨水作中和剂、补料液为75 g/L的葡萄糖、采用连续变速补料,活菌数提高到了2.56×10^(10)CFU/mL,是基础的MRS培养下的15.51倍。该研究建立了Lp.R3的高密度生产方法,为Lp.R3的工业化生产提供了技术支持。Lacticaseibacillus paracasei Lp.R3 is a probiotic strain with excellent functions.In order to realize its industrial application,high-density cultivation was carried out to increase the number of viable bacteria,the effects of medium composition,static culture conditions,and fermentation tank processes on the number of viable Lp.R3 bacteria were investigated by single factor experiments and orthogonal experiments optimization.The best result(4.75×10^(9)CFU/mL of viable bacteria number)was achieved when Lp.R3 cultured in the medium supplemented with 30 g/L glucose,50 g/L yeast extract,10 g/L anhydrous sodium acetate,0.1 g/L manganese sulfate,and 1 g/L Tween80 under the culture conditions of 1%inoculation level,pH7 and 35℃,which was 2.91 times greater than that cultured in MRS medium.In terms of the production in 5 L fermentation tanks,the best result(2.56×10^(10)CFU/mL of viable bacteria number)was given when L.p R3 fermented under constant pH of 5 neutralized by 25%ammonia,continuous variable feed of 75 g/L glucose,and agitation without aeration,which was 15.51 times greater than that cultured in MRS medium.In summary,the high-density production protocol of Lp.R3 was optimized in present study,providing a promising guide to accomplish large-scale production of Lp.R3.

关 键 词：副干酪乳杆菌 高密度培养 恒 PH 培养 工艺优化 活菌数 

分 类 号：TS201.3[轻工技术与工程—食品科学]