霍山石斛多糖硒化工艺的优化及对α-淀粉酶的抑制作用  

Selenization Optimization of Preparation Process of Polysaccharide from Dendrobium huoshanense and Its Inhibitory Effect onα-Amylase

作　　者：曹皖雪 李姣 陶强 范璇璇 陆继婷 陈乃富 陈乃东[1,2,3,4] CAO Wanxue;LI Jiao;TAO Qiang;FAN Xuanxuan;LU Jiting;CHEN Naifu;CHEN Naidong(College of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei 230012,China;Anhui Province Key Lab of Quality Evaluation and Improvement of Traditional Chinese Medicine,Lu'an 237012,China;College of Biotechnology and Pharmaceutical Engineering,West Anhui University,Lu'an 237012,China;Quality Evaluation and Quality Improvement of Chinese Medicine Lu'an Laboratory,Lu'an 237012,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]中药质量评价与品质提升安徽省重点实验室,安徽六安237012 [3]皖西学院生物与制药工程学院,安徽六安237012 [4]中药质量评价与品质提升六安市实验室,安徽六安237012

出　　处：《食品工业科技》2025年第9期206-214,共9页Science and Technology of Food Industry

基　　金：安徽省中药质量评价与品质提升科研创新团队(2022AH010090);安徽省高校协同创新中心项目(GXXT-2022-079);安徽省高等学校科学研究项目(2024AH051998)。

摘　　要：目的:优化霍山石斛多糖(Dendrobium huoshanense polysaccharide,DHP)硒化工艺并探讨DHP硒化前后对α-淀粉酶活性的抑制作用及机制。方法:通过单因素实验探究亚硒酸钠与DHP质量比、反应时间、硝酸浓度、反应温度对霍山石斛硒化多糖(Selenium-containing Dendrobium huoshanense polysaccharide,Se-DHP)中硒含量的影响。以硒含量为响应值,响应面优化Se-DHP的制备工艺。对比DHP和Se-DHP对α-淀粉酶的活性抑制率,并通过荧光猝灭实验探究其抑制机理。结果:DHP的最佳硒化工艺为:反应温度70℃、反应时间460 min、亚硒酸钠与DHP的质量比1:1、硝酸浓度为0.5%。在此条件下进行重复实验,得到Se-DHP硒含量为9223.51μg/g,与预测值9280.59μg/g接近。相较于DHP,Se-DHP对α-淀粉酶的抑制作用明显增强。荧光猝灭实验结果显示,DHP和Se-DHP对α-淀粉酶内源性荧光的猝灭作用均为静态猝灭。结论:本研究为制备高硒含量的Se-DHP提供一定的理论基础,为霍山石斛硒化多糖作为一种新的补硒制剂在药食领域的进一步应用提供参考。Objectives:This study aimed to optimize the selenization process of polysaccharides derived from Dendrobium huoshanense(DHP)and to investigate the inhibitory effects and mechanisms of both DHP and selenium-enriched DHP(Se-DHP)onα-amylase activity.Methods:To investigate the impact of factors such as the mass ratio of sodium selenite to DHP,reaction time,nitric acid concentration,and reaction temperature on the selenium content in Se-DHP,a single factor experiment was conducted.Optimization of the Se-DHP preparation conditions was achieved using response surface methodology,with selenium content as the response variable.Additionally,the inhibitory effects of DHP and Se-DHP onα-amylase activity were compared,and their inhibition mechanisms were examined through fluorescence quenching experiments.Results:The optimal conditions for selenization were identified as follows:reaction temperature of 70℃,reaction time of 460 minutes,a mass ratio of sodium selenite to DHP of 1:1,and a nitric acid concentration of 0.5%.Under these conditions,the selenium content in Se-DHP was 9223.51μg/g,closely matching the predicted value of 9280.59μg/g.Compared to DHP,Se-DHP demonstrated a significantly enhanced inhibitory effect onα-amylase.Fluorescence spectroscopy revealed that both DHP and Se-DHP exhibited static quenching mode forα-amylase.Conclusions:The findings provide a theoretical framework for the preparation of Se-DHP with high-selenium content and offer a reference for the potential use of selenium polysaccharides from DHP as a new selenium supplement in medicinal and nutritional applications.

关 键 词：硒化 霍山石斛多糖(Dendrobium huoshanense POLYSACCHARIDE DHP) 响应面法 Α-淀粉酶 

分 类 号：TS201.1[轻工技术与工程—食品科学]