羊肚菌多糖的体外消化、酵解特性及泡腾片制备  

In Vitro Digestion and Fermentation Characteristics of Mochella Polysaccharides and the Preparation of Effervescent Tablets

作　　者：吴丝雨 陈鸿 佘智煜 宋光明 邓利玲 赵祎 钟耕[1,3] WU Siyu;CHEN Hong;SHE Zhiyu;SONG Guangming;DENG Liling;ZHAO Yi;ZHONG Geng(College of Food Science,Southwest University,Chongqing 400715,China;Chongqing Medical and Pharmaceutical College,Chongqing 401331,China;Chongqing Key Laboratory of Speciality Food Co-built by Sichuan and Chongqing,Chongqing 400716,China)

机构地区：[1]西南大学食品科学学院,重庆400715 [2]重庆医药高等专科学校,重庆401331 [3]川渝共建特色食品重庆市重点实验室,重庆400716

出　　处：《食品工业科技》2025年第9期224-233,共10页Science and Technology of Food Industry

基　　金：农业产业融合发展“重庆渝十味中药材产业集群”项目([2024]102号)。

摘　　要：为探究羊肚菌多糖的体外消化及酵解特性并开发具有明确功效成分和含量的新型羊肚菌多糖泡腾片产品,本文探究羊肚菌多糖进行体外模拟唾液-胃肠消化和人粪酵解过程的变化,并通过大肠杆菌(Escherichia coli)和乳酸杆菌(Lactobacillus)的体外增殖试验评估其对肠道生态的影响。采用单因素实验并结合模糊数学感官评价法,以泡腾片的模糊感官评分为指标优化羊肚菌多糖泡腾片制备工艺。通过检测泡腾片水分含量、片重、厚度、崩解时限、发泡量及硬度等指标评价其品质。羊肚菌多糖模拟体外消化及酵解研究表明,其在唾液、胃和小肠液中稳定性较好,易被大肠肠道菌群降解并吸收利用,且具有益生活性,可作为益生元的潜在来源。体外增殖试验表明,羊肚菌多糖对常见的2种肠道细菌(大肠杆菌和乳酸杆菌)的生长有促进作用,可参与调节人体肠道微生态。实验得到泡腾片最佳配方:羊肚菌多糖10%、崩解剂65%、甜味剂1.5%、润滑剂0.5%。对所得泡腾片理化指标进行检测,均符合《中国药典》的规定。本研究证明了羊肚菌多糖具有益生活性,为羊肚菌多糖开发利用提供理论依据。In order to explore in vitro digestion and fermentation characteristics of Mochella polysaccharides and develop a new type of morel polysaccharide effervescent tablet with definite efficacy ingredients,in this study,the changes in simulated saliva-gastrointestinal digestion and human fecal fermentation was explored.Additionally,the impact of Mochella polysaccharides on intestinal ecology through in vitro proliferation experiments with Escherichia coli and Lactobacillus was evaluated.A single-factor experiment combined with fuzzy mathematics sensory evaluation was conducted to optimize the preparation process of Mochella polysaccharides effervescent tablets,using fuzzy sensory scores as the evaluation index.The quality of the effervescent tablets was evaluated by measuring their moisture content,weight,thickness,disintegration time,foaming capacity,and hardness.Simulated digestion and fermentation studies revealed that Mochella polysaccharides exhibited good stability in saliva,gastric,and intestinal fluids.They were readily degraded by intestinal microbiota and could be absorbed and utilized,highlighting their potential as a prebiotic source.In vitro proliferation experiments showed that Mochella polysaccharides promoted the growth of two common intestinal bacteria,Escherichia coli and Lactobacillus,thereby helping regulate human intestinal microecology.The optimal formulation of the effervescent tablets was determined to be 10%Mochella polysaccharides,65%disintegrant,1.5%sweetener,and 0.5%lubricant.The physicochemical properties of the resulting tablets met the standards set by the Chinese Pharmacopoeia.This study highlights the probiotic potential of Mochella polysaccharides and provides a theoretical foundation for their development and utilization.

关 键 词：羊肚菌多糖 体外消化 体外酵解 肠道微生物 泡腾片 

分 类 号：TS201.2[轻工技术与工程—食品科学]