一种快速高效测定乳粉和柿饼中α、β-胡萝卜素方法的建立  

Establishment of a rapid and efficient method for the determination ofα-andβ-carotene in milk powder and dried persimmons

作　　者：李国薇 张艳 张倩婷 强鑫 李璐 LI Guowei;ZHANG Yan;ZHANG Qianting;QIANG Xin;LI Lu(Fuping County Inspection and Testing Center(Shaanxi Sheep Milk Product Quality Supervision and Inspection Center),Weinan 711700)

机构地区：[1]富平县检验检测中心(陕西省羊乳产品质量监督检验中心),渭南711700

出　　处：《中国食品添加剂》2025年第4期131-138,共8页China Food Additives

基　　金：中外合作富平县特色羊乳制品质量安全控制研究;渭南市科技计划项目(2022ZDYFJH-47)。

摘　　要：通过对样品进行酶解、皂化、提取、氮吹、复溶,采用ZORBAX Eclipse PAH色谱柱(250 mm×4.6 mm,5μm)进行分离,以甲醇:乙腈:甲基叔丁基醚(95∶95∶10)为流动相,等度洗脱,外标法定量等,建立一种简单高效测定乳粉和柿饼中α、β-胡萝卜素的高效液相色谱方法。该方法测定的α-胡萝卜素在0.0048~9.65μg/mL,β-胡萝卜素在0.0051~10.12μg/mL浓度范围内线性关系良好,相关系数均大于0.999;α-胡萝卜素回收率在82.7%~104.3%之间,β-胡萝卜素回收率在80.3%~113.1%之间,精密度在0.68%~0.81%之间。本实验建立的方法前处理简单、回收率高、重复性好、结果准确可靠,适合乳粉和柿饼中α、β-胡萝卜素的同时测定。The samples were enzymolized,saponified,extracted,nitrogen evaporated and redissolved,then separated by ZORBAX Eclipse PAH column(250 mm×4.6 mm,5μm)with methanol:acetonitrile:methyl tert-butyl ether(95∶95∶10)as the mobile phase,and the samples were eluted by equal degree and quantified by external standard method.A simple and efficient HPLC method was established for the determination ofαandβ-carotene in milk powder and dried persimmon.In this method,α-carotene had good linearity in the concentration range of 0.0048 to 9.65μg/mL,andβ-carotene had good linearity in the concentration range of 0.0051 to 10.12μg/mL,with correlation coefficients greater than 0.999.The recoveries ofα-carotene ranged from 82.7%to 104.3%,the recoveries ofβ-carotene ranged from 80.3%to 113.1%,and the precision ranged from 0.68%to 0.81%.The method established in this experiment had the advantages of simple pretreatment,high recovery rate,good repeatability,accurate and reliable results,making it suitable for the simultaneous determination ofα-andβ-carotene in milk powder and dried persimmons.

关 键 词：乳粉 柿饼 胡萝卜素 高效液相色谱 

分 类 号：TS202.3[轻工技术与工程—食品科学]