茶树醛脱氢酶(ALDH)超家族的鉴定与表达分析  

Identification and Expression Analysis of the Aldehyde Dehydrogenases(ALDHs)Superfamily in Tea Plant(Camellia sinensis)

作　　者：刘丹丹 王雷刚 孙明慧 焦小雨 阮旭 吴琼 王文杰 LIU Dan-Dan;WANG Lei-Gang;SUN Ming-Hui;JIAO Xiao-Yu;RUAN Xu;WU Qiong;WANG Wen-Jie(Tea Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230001,China)

机构地区：[1]安徽省农业科学院茶叶研究所,合肥230001

出　　处：《农业生物技术学报》2025年第4期755-770,共16页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金联合基金(U23A20213);安徽省茶叶产业技术体系(AHCYJSTX-11);安徽省农业科学院成果转化项目(2024YL041)。

摘　　要：醛脱氢酶(aldehyde dehydrogenases,ALDHs)在植物非生物胁迫下的醛稳态中发挥着重要作用。本研究利用生物信息学方法鉴定茶树(Camellia sinensis)ALDH超家族成员,分析其编码的蛋白理化特性、染色体定位、基因结构、启动子顺式作用元件和系统进化关系等生物学信息;利用qRT-PCR技术分析CsALDH超家族成员在干旱、盐和低温胁迫下的表达模式。结果显示,在茶树基因组中鉴定到27个ALDH超家族成员,系统发育分析将其划分为10个家族。同一家族的成员具有相似的基序组成和基因结构。共线性与进化分析表明,CsALDHs基因在茶树基因组内发生基因复制事件,且在进化过程中经历了纯化选择作用。qRT-PCR结果显示,分别有8、10和9个候选CsALDHs基因被PEG、NaCl和低温诱导表达,尤其是CsALDH2B4和CsALDH3H2显著上调表达。本研究为深入研究茶树ALDH基因的功能提供了理论基础。Aldehyde dehydrogenases(ALDHs)play an important role in aldehyde stabilization under abiotic stress in plants.The members of ALDH superfamily in tea plant(Camellia sinensis)were identified by bioinformatics methods,and the biological information such as physicochemical properties,chromosome localization,gene structure,promoter cis-acting elements and phylogenetic relationship of the encoded proteins were analyzed.The expression patterns of the CsALDH superfamily members under drought,salt,and cold stresses ware analyzed by qRT-PCR.The results showed that a total of 27 ALDH superfamily members were identified in the C.sinensis genome,and phylogenetic analyses classified them into 10 families.Family members exhibited comparable motif composition and gene structure.The co-linearity analysis indicated that CsALDHs genes underwent gene duplication events in the C.sinensis genome,which experienced purifying selective pressures.The qRT-PCR results showed that 8,10 and 9 candidate CsALDHs genes were induced by PEG,NaCl and low temperature,respectively.In particular,CsALDH2B4 and CsALDH3H2 were significantly up-regulated.This study provides a theoretical foundation for further research on the functions of ALDH genes in tea plants.

关 键 词：茶树 醛脱氢酶 全基因组鉴定 系统进化 非生物胁迫 

分 类 号：S571.1[农业科学—茶叶生产加工]