双酶水解-DNS比色法测定白卡纸中淀粉含量的研究  

Determination of Starch in White Card Paper by Double Enzymolysis-DNS Colorimetric Method

作　　者：刘文婷 张胜华[1] 李陈巧 孙鹏 王鹏[3] 安俊健[3] LIU Wenting;ZHANG Shenghua;LI Chenqiao;SUN Peng;WANG Peng;AN Junjian(China Tobacco Hubei Industry Co.,Ltd.,Wuhan,Hubei Province,430040;Wuhan Hongjinlong Printing Co.,Ltd.,Wuhan,Hubei Province,430100;Hubei Key Lab of Green Light Industrial Materials,Hubei University of Technology,Wuhan,Hubei Province,430068)

机构地区：[1]湖北中烟工业有限责任公司,湖北武汉430040 [2]武汉红金龙印务股份有限公司,湖北武汉430100 [3]湖北工业大学绿色轻工材料湖北省重点实验室,湖北武汉430068

出　　处：《中国造纸》2025年第3期129-135,共7页China Pulp & Paper

基　　金：国家自然科学基金(32071722)。

摘　　要：为测定白卡纸中的淀粉含量,本研究选用α-淀粉酶和糖化酶对白卡纸进行双酶协同降解,采用3,5-二硝基水杨酸(DNS)比色法对酶解产物葡萄糖进行测定,进而计算白卡纸中的淀粉含量。在单因素实验分析研究的基础上,采用响应面分析法优化双酶水解-DNS比色法测定淀粉含量的最佳条件,建立数学模型,回归方程失拟误差不显著(P<0.000 1),理论值与测定值吻合(R^(2)为0.988 6);双酶水解-DNS比色法测定淀粉含量的最佳条件为:双酶添加量5 mg/mL,酶解时间75 min,酶解温度40℃。对该方法进行精密度和加标回收率测试,相对标准偏差为0.8%,加标回收率为96.2%~98.0%。To determine the starch content in white card paper,Glucoamylase andα-amylase were used to degrade the starch synergistical-ly.Glucose of the enzyme hydrolysis product in the solution was measured by 3,5-dinitrosalicylic acid colorimetry(DNS)colorimetry and the starch content in white card paper was calculated.Based on the single factor experiment,response surface method was carried out to in-vestigate the effects of key parameters,and the quadratic term model was established.The results of regression analysis presented a non-sig-nificant lack of fit(P<0.0001)and a high coefficient of determination value(R^(2)=0.9886)for the response surface model.The optimum de-termination conditions of starch using double enzymolysis-DNS colorimetry were double enzyme addition amount of 5 mg/mL,enzymatic time of 75 min,enzymatic temperature of 40℃.The relative standard deviations and standard recovery of the method were 0.8%and 96.2%~98.0%,respectively.

关 键 词：白卡纸 淀粉 Α-淀粉酶 糖化酶 测定 

分 类 号：TS762.2[轻工技术与工程—制浆造纸工程]