动物双歧杆菌动物亚种联合传统发酵剂对发酵乳体外降血糖与抗氧化特性的影响  

Effect of Bifidobacterium animal is subsp.animalis combined with traditional starter cultures on hypoglycemic and antioxidant properties of fermented milk in vitro

作　　者：冯贤丹 潘坤 李思宁[1] 唐善虎[1] 田木 FENG Xiandan;PAN Kun;LI Sining;TANG Shanhu;TIAN Mu(College of Food Science and Technology,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学食品科学与技术学院,成都610041

出　　处：《中国乳品工业》2025年第4期37-43,共7页China Dairy Industry

基　　金：中央高校基本科研业务费专项资金项目(ZYN2024147);四川省科技成果转化项目(2022ZHCG0017);四川省重大科技成果转化项目(2022ZHCG0129)。

摘　　要：文章探究不同发酵组合(发酵剂或底物)对发酵乳体外降血糖与抗氧化活性的影响,研究了动物双歧杆菌动物亚种(Bifidobacterium animalis supsp.animalis,Ba)与传统酸奶发酵剂(SL)共培养对各组发酵乳在低温贮藏期间α-淀粉酶抑制率、α-葡萄糖苷酶抑制率、DPPH·清除率、·OH清除率、Fe^(2+)螯合能力及还原能力的影响。结果表明,各组发酵乳α-淀粉酶抑制率、α-葡萄糖苷酶抑制率、DPPH·清除率、·OH清除率和还原能力在整个低温贮藏期内呈先升后降趋势,Fe^(2+)螯合能力呈相反趋势。发酵底物相同时,Ba联合SL发酵可显著提高发酵乳的α-淀粉酶抑制率、DPPH·清除率、·OH清除率、Fe^(2+)螯合能力和还原能力(P<0.05),但对α-葡萄糖苷酶抑制率无显著影响(P>0.05)。发酵剂相同时,各组发酵乳α-淀粉酶抑制率由高到低依次为发酵混合乳、发酵牦牛乳、发酵牛乳;α-葡萄糖苷酶抑制率、Fe^(2+)螯合能力和还原能力由高到低依次为发酵牛乳、发酵混合乳、发酵牦牛乳。结果表明,Ba联合SL共培养可提高发酵乳体外降血糖及抗氧化活性;相比于牦牛乳,牛乳和混合乳更适合作为发酵底物。To investigate the effects of different fermentation combinations(fermenter or substrate)on the hypoglycemic and antioxidant activities of fermented milk in vitro,the effects of co-culture of Bifidobacterium animalis ssp.Lactis(Ba)co-cultured with traditional yogurt fermenter(SL)onα-amylase inhibition,α-glucosidase inhibition,DPPH·scavenging,·OH-scavenging,Fe^(2+)chelating capacity and reducing capacity of each group of fermented milks during low temperature storage.The results indicated that theα-amylase inhibition,α-glucosidase inhibition,DPPH·scavenging,·OH scavenging and reducing capacity of fermented milks in each group showed an increasing and then a decreasing trend throughout the low-temperature storage period,and the Fe^(2+)chelating capacity showed an opposite trend.When the fermentation substitutes were the same,Ba combined with SL fermentation significantly increased theα-amylase inhibition,DPPH·scavenging,·OH scavenging,Fe^(2+)chelating capacity,and reducing capacity of fermented milk(P<0.05),but did not significantly affect theα-glucosidase inhibition(P>0.05).When the fermenting agent was the same,theα-amylase induction rate of each group of fermented milk was fermented mixed milk,fermented yak milk and fermented cow milk in descending order;theα-glucosidase inhibition rate,Fe^(2+)chelating ability and reducing ability were fermented cow milk,fermented mixed milk and fermented yak milk in descending order.The findings of this study showed that Ba combined with SL co-culture could enhance the hypoglycemic and antioxidant activities of fermented milk in vitro;cow's milk and mixed milk were more suitable as fermentation substrates compared to yak's milk.

关 键 词：发酵乳 共发酵 动物双歧杆菌 抗氧化活性 降血糖活性 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]