红枣多糖的降解红枣多糖的降解、结构表征及抗氧化活性结构表征及抗氧化活性  

Degradation,Structural Characterization and Antioxidant Activity of Jujube Polysaccharide

作　　者：易宇欣 薛地 宋年年 阿衣吐逊·阿布都外力 YI Yuxin;XUE Di;SONG Niannian;Aytursun Abuduwaili(College of Chemistry and Chemical Engineering,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China)

机构地区：[1]新疆农业大学化学化工学院,新疆乌鲁木齐830052

出　　处：《食品研究与开发》2025年第8期71-79,共9页Food Research and Development

基　　金：新疆农业大学高层次人才科研培育项目(2522GCCRC);新疆维吾尔自治区“三区人才”项目(2224SQRC);新疆维吾尔自治区自然科学基金青年基金项目(2022D01B89)。

摘　　要：该文经水提醇沉法制备红枣多糖(jujube polysaccharide,JP),利用H2O2‐VC法对红枣多糖进行降解得到3种降解产物(JP‐30、JP‐60、JP‐90),并对原多糖与3种降解产物进行结构表征和抗氧化活性的对比研究。结果表明:JP提取率达(6.57±0.33)%;JP及其降解产物的糖含量及糖醛酸含量为54.58%~60.69%及60.95%~66.74%,均由不同物质的量比的鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖及半乳糖组成;JP、JP‐30、JP‐60、JP‐90的分子量分别为81.46、13.50、12.65、11.92 kDa。圆二色谱、扫描电子显微镜、刚果红染色结果表明降解会显著影响多糖高级结构。同时,降解处理可以显著改善多糖的抗氧化活性,其中,JP‐90清除ABTS+自由基及DPPH自由基的能力最强,半抑制浓度分别为0.0600 mg/mL和0.0044 mg/mL。Jujube polysaccharide(JP)was prepared by water extraction and alcohol precipitation method.The products of JP including JP⁃30,JP⁃60 and JP⁃90 were prepared by the H2O2⁃VC method.The structural charac⁃terization and antioxidant activities of the native polysaccharide and its degraded products were comparatively investigated.According to the result,the yield of JP was(6.57±0.33)%.The carbohydrate and uronic acid con⁃tent of the JP and its degradation products were 54.58%-60.69%and 60.95%-66.74%,mainly composed of rhamnose,arabinose,xylose,glucose and galactose with different molar ratios.The molecular weights of JP,JP⁃30,JP⁃60,and JP⁃90 were 81.46,13.50,12.65,11.92 kDa,respectively.The results of infrared spectroscopy,circular dichroism,and scanning electron microscopy showed that degradation could significantly affect the se⁃nior structure of the polysaccharide.Meanwhile,the degradation could significantly improve the antioxidant ac⁃tivity of the polysaccharide,and JP⁃90 had the strongest scavenging ability against ABTS+and DPPH free radi⁃cals with half⁃inhibitory concentrations of 0.0600 and 0.0044 mg/mL,respectively.

关 键 词：红枣 多糖 降解 结构表征 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]