产普鲁兰酶海洋芽孢杆菌A2的筛选鉴定及酶学性质与应用  

Screening,Identification,Enzymatic Property Characterization,and Applications of Marine Bacillus sp.A2 Producing Pullulanase

作　　者：邵如雪 相卜瑗 周俊兰 汪明奥 亢新鑫 王淑军 SHAO Ruxue;XIANG Boyuan;ZHOU Junlan;WANG Ming′ao;KANG Xinxin;WANG Shujun(Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China;Jiangsu Provincial Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China)

机构地区：[1]江苏海洋大学江苏省海洋生物资源与环境重点实验室,江苏连云港222005 [2]江苏海洋大学江苏省海洋生物技术重点实验室,江苏连云港222005

出　　处：《食品研究与开发》2025年第7期174-181,共8页Food Research and Development

基　　金：国家重点研发计划项目(2022YFC2805101);江苏省科研实践创新项目(SJCX22-1659)。

摘　　要：该研究从海泥样品中筛选产普鲁兰酶的微生物。采用透明圈法从青岛海泥中筛选到产普鲁兰酶的菌株A2,通过菌株形态特征和16S rDNA分析,鉴定菌株A2为芽孢杆菌属(Bacillus sp.),其生长的最适碳源、氮源、生长温度和pH值分别为酵母浸膏、大豆蛋白胨、40℃和7.0;产酶的最适碳源、氮源、温度和初始pH值分别为蔗糖、大豆蛋白胨、45℃和7.0。普鲁兰酶的最适温度和初始pH值分别为35℃和6.0,在35~50℃、pH5.0~9.0范围具有良好的稳定性;Ba^(2+)、NH_(4)^(+)、Zn^(2+)、K^(+)、Mg^(2+)、Cu^(2+)、Ca^(2+)和Na^(+)等金属离子对酶活影响小。用该酶水解不同种类淀粉,可观察到玉米淀粉表面形成均匀的多孔形态,该酶可用于制备玉米多孔淀粉。The microorganisms producing pullulanase were screened from marine samples.A strain A2 producing pullulanase was isolated from the sea mud collected from Qingdao by the transparent circle method.Strain A2 was identified as Bacillus sp.based on the morphological characteristics and 16S rDNA sequence.The optimum carbon source,nitrogen source,temperature,and pH value for the growth of the strain were yeast extract,soybean peptone,40℃,and 7.0,respectively,and those for enzyme production of the strain were sucrose,soybean peptone,45℃,and 7.0,respectively.The optimum temperature and pH value of pullulanase were 35℃and 6.0,respectively,and the pullulanase demonstrated good stability within the ranges of 35-50℃and pH5.0-9.0.Metal ions such as Ba^(2+),NH_(4)^(+),Zn^(2+),K^(+),Mg^(2+),Cu^(2+),Ca^(2+),and Na^(+)had little effects on the enzyme activity.When different kinds of starch were hydrolyzed by this enzyme,uniform porous morphology was observed on the surface of corn starch.The enzyme can be used to prepare porous starch of maize.

关 键 词：海洋细菌 筛选鉴定 普鲁兰酶 酶学性质 玉米多孔淀粉 

分 类 号：Q93[生物学—微生物学]