成都市预包装即食冷藏熟肉制品中单核细胞增生李斯特氏菌污染特征和致病潜力研究  

Analysis of the contamination characteristics and pathogenic potential of Listeria monocytogenes in pre-packaged and refrigerated ready-to-eat cooked meat products in Chengdu City

作　　者：刘肖 孙宏虎 刘丽莎 王翔[3] 李少艇 张鑫昊 李澜奇 白莉 Liu Xiao;Sun Honghu;Liu Lisha;Wang Xiang;Li Shaoting;Zhang Xinhao;Li Lanqi;Bai Li(Key laboratory of Food Safety Risk Assessment of National Health Commission,China National Centre for Food Safety Risk Assessment,Beijing 100021,China;Irradiation Preservation Key Laboratory of Sichuan Province,Chengdu Institute of Food Inspection,Chengdu 610045,China;School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;School of Biomedical and Pharmaceutical Sciences,Guangdong University of Technology,Guangzhou 510006,China)

机构地区：[1]国家食品安全风险评估中心国家卫生健康委食品安全风险评估重点实验室,北京100021 [2]成都市食品检验研究院辐照保藏四川省重点实验室,成都610045 [3]上海理工大学健康科学与工程学院,上海200093 [4]广东工业大学生物医药学院,广州510006

出　　处：《中华预防医学杂志》2025年第4期443-450,共8页Chinese Journal of Preventive Medicine

摘　　要：目的分析成都市预包装即食冷藏熟肉制品中单核细胞增生李斯特氏菌(LM)的污染特征和致病潜力。方法2022年9月到2023年5月,从成都市6个区县采集预包装即食冷藏熟肉制品,开展LM定性和定量检测,对分离菌株开展全基因组测序,选取不同ST型菌株进行细胞黏附和侵袭实验,结果以黏附率和侵袭率表示,采用单因素方差分析进行组间比较,Dunnett t检验进行两两比较。结果共采集145份样品,其中29份样品检出并分离到LM,总检出率为20.00%,卤肉三拼检出率最高(68.18%),9份(31.03%)样品LM污染量大于100.00 MPN/g。29株LM分别属于7个ST型别,包括ST 3(27.59%)、ST 8(17.24%)和ST 87(13.79%)等中高毒力菌株,其中ST 87的菌株携带毒力岛-4(LIPI-4),为高毒力菌株。部分中高毒力ST 8和ST 87的菌株与临床患者菌株亲缘关系较近,且部分相同ST型的LM分离株在同一生产厂家不同时期内,SNP差异较小(1~6)。体外细胞实验显示高毒力ST 87菌株对Caco-2细胞的黏附和侵袭能力最强。结论成都市预包装即食冷藏熟肉制品LM污染率较高,部分样品污染水平高且携带中高毒力菌株,对人体存在潜在健康风险,部分厂家生产加工环节LM持续残留。ObjectiveTo analyze the contamination characteristics and pathogenic potential of Listeria monocytogenes(LM)in pre-packaged and refrigerated ready-to-eat cooked meat products in Chengdu City.MethodsFrom September 2022 to May 2023,pre-packaged and refrigerated ready-to-eat cooked meat products were collected from six districts and counties in Chengdu City.Qualitative and quantitative determination of LM was performed on these samples.Whole genome sequencing was carried out on the isolated strains.Different ST strains were selected for cell adhesion and invasion experiments.The results were expressed as adhesion rate and invasion rate.One-way ANOVA was used for comparison between groups,and the Dunnett t-test was used for pairwise comparison.ResultsA total of 145 samples were collected,and LM was detected and isolated in 29 samples.The total detection rate was 20.00%,and the detection rate of braised pork was the highest(68.18%).The contamination level of LM in 9 samples(31.03%)was greater than 100.00 MPN/g.The 29 strains of Listeria monocytogenes belonged to 7 STs,including ST 3(27.59%),ST 8(17.24%)and ST 87(13.79%).The strain of ST 87 carried Listeria pathogenicity islands 4(LIPI-4),which was a highly virulent strain.The medium and high virulence strains accounted for 79.31%.Some moderately virulent and highly virulent strains of ST 8 and ST 87 were closely related to clinical patient strains.Some LM isolates of the same ST type had little SNP differences(1-6)in the same manufacturer at different stages.In vitro cell experiments showed that the highly virulent strain ST 87 had the strongest adhesion and invasion ability towards Caco-2 cells.ConclusionThe pre-packaged and refrigerated cooked meat products have a high contamination rate.Some samples have high contamination levels and carry medium and high virulence strains,posing potential health risks to human beings.LM residues continue to persist in some manufacturers′production and processing stages.

关 键 词：监测 单核细胞增生李斯特氏菌 即食熟肉制品 全基因组测序 黏附和侵袭 

分 类 号：R155.3[医药卫生—营养与食品卫生学]