Zebrafish optic nerve regeneration involves resident and retinal oligodendrocytes  

作　　者：Cristina Pérez-Montes Rosalía Hernández-García Jhoana Paola Jiménez-Cubides Laura DeOliveira-Mello Almudena Velasco Rosario Arévalo Marina García-Macia Adrián Santos-Ledo 

机构地区：[1]Institute of Neuroscience of Castilla y León(INCyL),Salamanca,Spain [2]Department of Human Anatomy and Histology,University of Salamanca,Salamanca,Spain [3]Institute for Biomedical Research of Salamanca(IBSAL),Salamanca,Spain [4]Department of Anatomy,Faculty of Medicine,University of Helsinki,Helsinki,Finland [5]Department of Cell Biology and Pathology,University of Salamanca,Salamanca,Spain [6]Institute of Functional Biology and Genomics(IBFG),University of Salamanca/CSIC,Salamanca,Spain [7]Department of Biochemistry and Molecular Biology,University of Salamanca,Salamanca,Spain

出　　处：《Neural Regeneration Research》2026年第2期811-820,共10页中国神经再生研究(英文版)

基　　金：supported by the Lanzadera TCUE and C2 program(Universidad de Salamanca)(to ASL);the Spanish National Research Council(CSIC)funded by the Junta de Castilla y León and co-financed by the European Regional Development Fund(ERDF“Europe drives our growth)”:Internationalization Project“CL-EI-2021-08-IBFG Unit of Excellence”,Grant(PID2022-138478OA-100)funded by MICIU/AEI/10.13039/501100011033 and,by FEDER,UE(to MGM);Junta de Castilla y León(SA225P23)and Gerencia Regional de Salud(2701/A1/2023)(to AV);the Plan Especial Grado Medicina(USAL)(to CPM);a Ramón y Cajal researcher:Grant RYC2021-033684-I funded by MICIU/AEI/10.13039/501100011033 and,by European Union NextGenerationEU/PRTR.

摘　　要：The visual system of teleost fish grows continuously,which is a useful model for studying regeneration of the central nervous system.Glial cells are key for this process,but their contribution is still not well defined.We followed oligodendrocytes in the visual system of adult zebrafish during regeneration of the optic nerve at 6,24,and 72 hours post-lesion and at 7 and 14 days post-lesion via the sox10:tagRFP transgenic line and confocal microscopy.To understand the changes that these oligodendrocytes undergo during regeneration,we used Sox2 immunohistochemistry,a stem cell marker involved in oligodendrocyte differentiation.We also used the Click-iT™Plus TUNEL assay to study cell death and a BrdU assay to determine cell proliferation.Before optic nerve crush,sox10:tagRFP oligodendrocytes are located in the retina,in the optic nerve head,and through all the entire optic nerve.Sox2-positive cells are present in the peripheral germinal zone,the mature retina,and the optic nerve.After optic nerve crush,sox10:tagRFP cells disappeared from the optic nerve crush zone,suggesting that they died,although they were not TUNEL positive.Concomitantly,the number of Sox2-positive cells increased around the crushed area,the optic nerve head,and the retina.Then,between 24 hours post-lesion and 14 days post-lesion,double sox10:tagRFP/Sox2-positive cells were detected in the retina,optic nerve head,and whole optic nerve,together with a proliferation response at 72 hours post-lesion.Our results confirm that a degenerating process may occur prior to regeneration.First,sox10:tagRFP oligodendrocytes that surround the degenerated axons stop wrapping them,change their“myelinating oligodendrocyte”morphology to a“nonmyelinating oligodendrocyte”morphology,and die.Then,residual oligodendrocyte progenitor cells in the optic nerve and retina proliferate and differentiate for the purpose of remyelination.As new axons arise from the surviving retinal ganglion cells,new sox10:tagRFP oligodendrocytes arise from residual oligodendrocyte p

关 键 词：cell death OLIGODENDROCYTES optic nerve proliferation regeneration Sox10 SOX2 visual system ZEBRAFISH 

分 类 号：R741[医药卫生—神经病学与精神病学]