LC-MS/MS评价银耳中米酵菌酸的低温等离子体降解效果  

Evaluation of the Degradation Effect of Bongkreic acid by Low-Temperature Plasma in Tremella with Liquid Chromatography-Tandem Mass Spectrometry

作　　者：肖巾秀 夏微涵 王宗义 何世慧 张巍青 孙运金 XIAO Jinxiu;XIA Weihan;WANG Zongyi;HE Shihui;ZHANG Weiqing;SUN Yunjin(Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticides,College of Food Sciences and Engineering,Beijing University of Agriculture,Beijing 102206;Guangzhou Hexin Instrument Co.,Ltd.,Guangzhou 510535)

机构地区：[1]北京农学院食品科学与工程学院,农产品有害微生物及农残安全检测与控制北京市重点实验室,北京102206 [2]广州禾信仪器股份有限公司,广州510535

出　　处：《食品工业》2025年第3期270-274,共5页The Food Industry

基　　金：北京农学院教改重点项目-服务乡村振兴专业能力实训课程体系的构建与教学实践(5046516654/149)。

摘　　要：建立银耳中米酵菌酸(BA)的液相色谱-串联质谱检测方法,进而研究低温等离子体活化空气和活化水对BA的降解效果。银耳经粉碎,以BA-^(13)C_(28)为内标,经氨水-甲醇溶液提取,MAX型固相萃取小柱净化,液相色谱-串联质谱(LC-MS/MS),多反应监测(MRM)模式进行检测。将低温等离子体活化气体通入水中制备获得电导率为500μS/cm的活化水,对活化水中BA的降解情况和活化水降解活力的保持情况进行评价。将低温等离子体活化气体直接作用于银耳,评价其对银耳中BA的降解情况。优化的检测方法对BA分离效果良好,方法线性良好,R^(2)>0.999,水溶液中的检出限(LOD)和定量限(LOQ)分别为5 ng/mL和16 ng/mL,银耳粉中的LOD和LOQ分别为25 ng/g和82 ng/g。银耳中BA的加标回收率为89.40%~100.5%,S_(RSD)为5.64%~10.9%。在活化水溶液中BA迅速降解超过90%,12 h后超过99.96%。室温敞口、封口储存的活化水BA降解活力无差别,在监测的23 d内活力保持不变。活化气体、活化水直接处理人为BA污染的银耳样品,BA的降解率为30%~50%。试验建立的BA检测方法、灵敏可靠,为相关试验研究提供了有力工具。低温等离子对溶液中的BA有极强的降解能力,但在银耳中,受介质的影响,无论活化气体还是活化水,对BA的降解能力大幅下降。试验为银耳BA污染防治和降解技术的开发提供了科学依据和新的思路。To establish a liquid chromatography-tandem mass spectrometry method for the determination of Bongkreic acid(BA)in tremella,and then to study the degradation effect of air and water activated by low temperature plasma.Tremella was crushed,extracted by ammonia-methanol solution with BA-^(13)C_(28) as the internal standard,purified by MAX solid phase extraction column,and detected by liquid chromatogram-tandem mass spectrometry(LC-MS/MS)and multiple reaction monitoring(MRM)mode.Activated water with a conductivity of 500μS/cm was prepared by injecting low-temperature plasma activated gas into water.The degradation of BA in activated water and the retention of the degradation activity of activated water were evaluated.The degradation of BA in tremella by low temperature plasma activated gas was evaluated.The optimized detection method had good effect on BA separation,and the method was linear with R^(2)>0.999.The limit of detection(LOD)and limit of quantitation(LOQ)in aqueous solution were 5 ng/mL and 16 ng/mL,respectively.The LOD and LOQ in tremella powder were 25 ng/g and 82 ng/g,respectively.The recoveries of BA and S_(RSD)were 89.40%-100.5%and 5.64%-10.9%respectively.BA rapidly degraded more than 90%in the activated aqueous solution,and more than 99.96%after 12 h.There was no difference in the degradation activity of activated water BA in open and sealed storage at room temperature,and the activity remained unchanged within 23 days of monitoring.The degradation rate of BA was 30%-50%when activated gas and activated water directly treated the white fungus samples polluted by artificial BA.The BA detection method established in the experiment is sensitive and reliable,which provides a powerful tool for related experimental research.Low temperature plasma has a strong ability to degrade BA in solution,but in tremella,the degradation ability of BA decreases greatly under the influence of the medium,whether activated gas or activated water.The experiment provided scientific basis and new ideas for the development of

关 键 词：低温等离子体 银耳 米酵菌酸 液相色谱-串联质谱法 降解 

分 类 号：TS201.2[轻工技术与工程—食品科学]