降解展青霉素乳酸菌的筛选及其动力学研究  

Screening of lactic acid bacteria for degradation of patulin and its kinetic study

作　　者：周晨阳 陆胜民 杨虎清[1] ZHOU Chenyang;LU Shengmin;YANG Huqing(School of Food and Health,Zhejiang A&F University,Hangzhou 311300,China;State Key Laboratory for Quality and Safety of Agro-Products,Zhejiang Key Laboratory of Intelligent Food Logistics and Processing,Institute of Food Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江农林大学食品与健康学院,浙江杭州311300 [2]浙江省农业科学院食品科学研究所,农产品质量安全全国重点实验室,全省生鲜食品智慧物流与加工重点实验室,浙江杭州310021

出　　处：《浙江农业学报》2025年第4期943-953,共11页Acta Agriculturae Zhejiangensis

基　　金：省部共建农产品质量危害因子与风险防控国家重点实验室资助项目(2021DG700024-ZZ202210)。

摘　　要：展青霉素(patulin,PAT)是威胁果蔬产业和消费者健康的潜在风险因素,降解展青霉素的物理化学方法存在污染环境、效果不明显等弊端,而生物降解逐渐成为主流。本研究从发酵产品中分离筛选乳酸菌,探究试验菌株对PAT降解的效果和影响条件。结果表明:从12株乳酸菌中筛选出1株PAT降解优势菌株,经生物学鉴定为Levilactobacillus brevis。在37℃、pH值>3、菌液浓度为4×10^(8)mL^(-1)条件下,该乳酸菌对PAT(1~100μg·mL^(-1))的降解率高达95%以上。动力学分析表明,该乳酸菌高效降解的时间发生在0~6 h,符合一级动力学方程[ln(C_(0)/C_(t))=0.47205 t+0.01274,R^(2)=0.99],且发现1种潜在的降解产物,并鉴定其为hydroascladiol。综上,该试验菌株对降解PAT具有良好的发展潜力。Patulin(PAT)is a potential risk factor that threatens the fruit and vegetable industry and health of consumers.Existing physical and chemical methods have drawbacks such as environmental pollution and insufficient effects,while biodegradation is gradually becoming mainstream.Lactic acid bacteria were isolated from fermented products to explore the effectiveness and influencing conditions of experimental strains on PAT degradation in this study.A dominant strain was screened out from 12 strains of lactic acid bacteria after PAT degradation effect testing,and was identified as Levilactobacillus brevis by biologic identification.Under the conditions of temperature 37℃,pH value>3,and bacterial solution concentrations of 4×10^(8)mL^(-1),more than 95%of PAT in concentration of 1~100μg·mL^(-1)was degraded.Kinetics analysis indicated that its efficient degradation occurred within 0-6 h,which followed the first-order kinetics with its equation being ln(C_(0)/C_(t))=0.47205 t+0.01274,R^(2)=0.99,and one potential degradation product,identified as hydroascladiol,was found.In summary,the experimental strain has good development potential for degrading PAT.

关 键 词：展青霉素 乳酸菌 降解动力学 降解产物 

分 类 号：TS201.3[轻工技术与工程—食品科学]