高效液相色谱法检测小麦、大米中赭曲霉毒素A  

Determination on ochratoxin A(OA) in samples of wheat and rice by high performance liquid chromatography

作　　者：谢妮[1] 徐强[2] 吕相征[1] 蔡原[1] 

机构地区：[1]中国医科大学公共卫生学院,沈阳110001 [2]沈阳师范大学化生分院

出　　处：《中国公共卫生》2003年第2期210-211,共2页Chinese Journal of Public Health

摘　　要：目的　建立赭曲霉毒素A的高效液相色谱检测方法 ;了解沈阳地区小麦、大米中赭曲霉毒素A的污染状况。方法　利用液 -液分离的前处理技术 ,选用C18反相柱 (2 5 0× 4 6mm) ,以乙腈∶0 0 0 8mol/L磷酸 =5 6∶44为流动相 ,流速 1ml/min荧光检测器 (激发波长 3 3 8nm ,发射波长 45 5nm) ,柱温 3 0℃。结果　实验所得回收率和精密度以及确证试验的结果均令人满意。结论　建立了用高效液相色谱法检测赭曲霉毒素A的方法 ,用本方法检测的 62份小麦、大米样品中 ,有 1份小麦样品为阳性 。Objective To build a method to deter mine OA in the sample with HPLC.Methods Chromatography conditions:C 18 column(250×4 6mm),mobile phase was acetonitrile 0 008 mol/L phosphoric acid(56∶44,v/v),fluorescence detector(excitation wavelength 338 nm;emission wavelength 455 nm) termperature of column:30 ℃.Results The recovery rate and precision was satisfactory.Conclusion This study established HPLC method to deter mine OA.One of sixty-two samples of wheat and rice was polluted by OA,which indicated contamination rate of cereal by OA was low.

关 键 词：高效液相色谱法 检测 小鼠 大米 赭曲霉毒素A 

分 类 号：R155.52[医药卫生—营养与食品卫生学]