NO mediates ginsenoside Rg_1-induced long-term potentiation in anesthetized rats  

一氧化氮介导麻醉大鼠人参皂苷Rg_1诱发长时程增强(英文)

作　　者：王晓英[1] 张均田[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所药理研究室,北京中国100050

出　　处：《Acta Pharmacologica Sinica》2001年第12期1099-1102,共4页中国药理学报（英文版）

基　　金：National Key Program on Basic Research and Development, No g1998051109

摘　　要：AIM: To investigate the effect of ginsenoside Rg1 on synaptic transmission in anesthetized rats and the effect of NOS inhibitor, 7-nitroindazole (7-NI), on long-term potentiation (LTP) induced by Rgj. METHODS: Extracellular recording technique was used to record the population spike (PS) in the dentate gyrus (DG) of anesthetized rats. Drug or vehicle injections were delivered via a cannula in the lateral cerebral ventricle. RESULTS: Rg1 (10 and 100 nmol/L) enhanced the basic synaptic transmission and the magnitude of LTP induced by high frequency stimulation (HFS). Selective nNOS inhibitor 7-NI (5 nmol) icv could inhibit the induction of perforant path-dentate gyrus LTP elicited by Rg1( P < 0.05), and L-arginine 250 g/L ip prevented the action of 7-nitroindazole (P < 0.05). CONCLUSION: Rg1-accelerated synaptic transmission and nitric oxide produced by nNOS played a role in the induction of PP-DG LTP in anesthetized rats.目的:研究人参皂苷Rg_1对麻醉大鼠突触传递效能的影响及作用机制。方法:应用细胞外微电极记录技术,记录麻醉大鼠海马齿状回颗粒细胞群体峰电位(PS)。结果:Rg_1(10,100nmol/L)提高麻醉大鼠的基础突触传递,诱导海马齿状回突触传递长时程增强(LTP),并提高高频刺激所诱导的LTP。选择性NOS抑制剂7-硝基吲唑(7-NI,5nmol)侧脑室注射可明显抑Rg_1所诱导的齿状回LTP,对基础突触传递无明显影响。L-Arg(250mg/kg,ip)可拮抗7-NI对Rg_1诱导LTP的抑制作用(P<0.05)。结论:Rg_1显著促进麻醉大鼠的突触传递效能,由神经元型NOS(nNOS)所产生的NO参与了Rg_1对齿状回LTP的诱导过程。

关 键 词：GINSENG SAPONINS long-term potentia-tion synaptic transmission nitric oxide INDAZOLES HIPPOCAMPUS dentate gyrus ARGININE 

分 类 号：R285.5[医药卫生—中药学]