Determination of idebenone in plasma by HPLC/MS  

HPLC/MS法测定血浆艾地苯醌(英文)

作　　者：胡蓓[1] 江骥[1] 谢宏红[1] 

机构地区：[1]中国协和医科大学北京协和医院临床药理中心

出　　处：《Acta Pharmacologica Sinica》2000年第4期306-308,共3页中国药理学报（英文版）

摘　　要：AIM: To develop a sensitive method for the analysis of idebenone [ Ide, 6- (10-hydroxydecyl) -2, 3-dimethoxy-5-methyl-p-benzoquinone] in human plasma. METHODS : The concentrations in plasma were determined by a high performance liquid chromatography-mass spectrome-try coupled with an atmosphere pressure chemical impact ion source method (LC/MS-APCI). RESULTS: The chromatograms indicated a good separation of the analytes and there was no interference by other compounds. The coefficients of determination of the calibration curves were above 0.999. The calibration range was 20 - 600 μg· L-1. Peak plasma idebenone concentration (Cmax) of (316±85) μg·L+1 was achieved within (96 ±34) min (Tpeak) after an oral dose of 30 mg. Lower limit of quantitation of the method for Ide determination was 20 μg·L-1. CONCLUSION: The advantages of using LC/MS-APCI technique include better sensitivity, higher selectivity, and less time consumption, compared with HPLC method.目的:建立测定艾地苯醌(Ide)血药浓度的灵敏方法。方法:HPLC/MS联用仪测定Ide血药浓度。离子源采用大气压化学电离源(APCI),MS扫描方式为选择离子检测(SIR)。结果:血浆中物质在本试验的LC/MS条件下对Ide和内标QSA-9的分离测定无任何影响。血浆中Ide浓度在20-600μg·L^(-1)范围内,浓度与峰面积有良好的线性关系,方法的最低检测浓度以s/n=7:1计为20μg·L^(-1)。结论:本法具有非常强的特异性,对HPLC柱分离效率的要求不高,分析所需时间短。

关 键 词：IDEBENONE PHARMACOKINETICS high pressure liquid chromatography mass spectrum analysis clinical pharmacology BENZOQUINONES 

分 类 号：R96[医药卫生—药理学]