高产谷胱甘肽的酵母融合菌株的选育及其培养条件的研究  

The Breeding of High Glutathione-producing Strain and Optimization of Culture Condition

作　　者：刘娟[1] 何秀萍[2] 王雅琴[1] 刘春秀[2] 孔英俊[1] 张博润[2] 

机构地区：[1]北京化工大学,北京100029 [2]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》2003年第1期99-103,共5页Acta Microbiologica Sinica

摘　　要：通过初筛、单倍体分离、诱变及原生质体融合技术选育到一株谷胱甘肽产量明显提高的融合菌株ZJF 71 ,其谷胱甘肽产量分别为亲株的 1 .5 9和 1 .42倍。并对其培养条件进行了研究 ,结果表明碳源、装液量和培养时间对融合菌株ZJF 71生产谷胱甘肽的影响较为显著。在优化的培养条件下 ,发酵液中谷胱甘肽总量达到 1 85 .3mg L ,为初始培养条件下的 2 .8倍。经遗传稳定性分析 ,证明融合菌株ZJF - 71是遗传稳定的。High glutathione-producing strain ZJF-71 was constructed by primary screening,isolation of haploid, mutagenesis and protoplasts fusion. The yield of glutathione of the fusant ZJF-71 is 1.59 and 1.42 times that of the parental strains Y64 and Y247, respectively. The factors that affected the biomass and glutathione content of the fusant ZJF-71 were also tested. The highest level of glutathione was obtained in 32 h at 30℃ and 200 r/min, when 30 mL of culture in 250- mL shake flasks was incubated in fermentation medium which contained (w/v): 6% cane sugar, 1% peptone, 1% yeast extract, and 2mmol/L cysteine. Glutathione yield under the optimal fermentation condition showed a 2.8-fold improvement over that of the initial condition. The fusant ZJF-71 is stable in genetic by analysis of genetic stability.

关 键 词：高产谷胱甘肽 酵母融合菌株 选育 培养条件 

分 类 号：Q939.9[生物学—微生物学]