纤维素酶产生菌的筛选及其固态发酵初步研究  

Preliminary studies on the filtration of cellulase producing fungi and its solid-state ferm entation

作　　者：叶生梅[1] 薛正莲[1] 王岚岚[1] 

机构地区：[1]安徽工程科技学院生物化学系,安徽芜湖241000

出　　处：《安徽理工大学学报（自然科学版）》2003年第1期57-59,共3页Journal of Anhui University of Science and Technology:Natural Science

基　　金：安徽省教育厅自然科学基金资助项目(2003kj039)

摘　　要：用预处理的天然纤维素为碳源,从土壤中定向筛选出8株产纤维素酶能力较强的真菌,选取其中CMC糖化力(CMCase)和滤纸糖化力(FPA)均较高的JZ-H进行固态发酵实验,初步研究发现:该菌以杂草为碳源、(NH4)2SO4和蛋白胨为氮源、500mL三角瓶装干料10g、自然pH、30℃培养72h,发酵物的CMCase达128.5IU/g,FPA达75.8U/g。Eight fungi of high cellulase production were screened from soil with pretreated natural cellulose for carbon resource. JZH with high CMCase and FPA was selected for solidstate fermentation experiment. The authors found that the leavening CMCase reached 128.5 IU/g and FPA 75.8 U/g when the fungi, with dry grass as carbon resource and (NH4)2SO4 and peptone as the nitrogen resource, were cultivated for 72 h under the condition of natural pH, 30 ℃ temperature and 10 g dry grass in 500 ml triangle bottle.

关 键 词：定向筛选 固态发酵 纤维素酶 纤维素 培养基 CMC 

分 类 号：TQ925[轻工技术与工程—发酵工程]