高效液相色谱法测定乳粉中维生素A,D  

Determination of Vitamin A and D in dairy product by high perform ance liquid chromato graphy

作　　者：刘波[1] 郎昭滨 殷晓红[1] 邹旭东[1] 王克新[1] 

机构地区：[1]国家乳制品质量监督检验中心,黑龙江哈尔滨150086

出　　处：《中国乳品工业》2003年第2期36-38,共3页China Dairy Industry

摘　　要：研究了高效液相色谱法,同时测定牛乳中维生素A,D。乳粉样品通过皂化、萃取,提取液通过aminocyano色谱柱净化、silica色谱柱分离,紫外检测器,外标定量法,同时测定出样品中维生素A,D。本法最低检出限VA为1.1×10-8g,VD为2.5×10-8g;变异系数VA为1.917%,VD为5.518%;平均回收率VA为96.3%,VD为90.2%。This is a method for determination of Vitamin A and D in dairy product by HPLC.Samp le is saponified and ether extract is in jected for clean-up on t he amino-cya no LC column and quantif ied on the silica LC column and is dete rmined by UV de tector.It is quantified by extern al standard.It is determined Vitami n A and D of sample in the same time.The minimum detectable value of Vita min A is 1. 1×10-8 g and Vitamin D is 2.5×10-8 g.The variation coefficient (CV%)of Vitamin A is 1.917%and Vitamin D is 5.518%.The recovery of Vitamin A is 96.3%,and Vitamin D is 9 0.2%.

关 键 词：高效液相色谱法 测定 乳粉 维生素A 维生素D 牛乳 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]