胶质母细胞瘤PTEN基因突变和ki-67表达的研究  

PTEN GENE MUTATION AND Ki-67 EXPRESSION IN GLIOBLASTOMAS

作　　者：屈洪涛[1] 刘运生[1] 肖亚飞[2] 梁有明[1] 

机构地区：[1]中南大学湘雅医院神经外科,长沙410078 [2]南华大学附一医院

出　　处：《中国现代医学杂志》2003年第8期37-39,47,共4页China Journal of Modern Medicine

摘　　要：目的 :研究胶质母细胞瘤中PTEN基因突变及Ki- 6 7表达情况 ,并探讨其与两者之间的关系。方法 :对10 2例胶质瘤 (包括 4 2例胶质母细胞瘤 )石蜡切片中PTEN基因进行聚合酶链反应～单链构象多态性分析(PCR -SSCP) ,检测PTEN基因突变。同时采用免疫组化S -P法 ,检测胶质瘤中Ki- 6 7的表达。结果 :4 2例胶质母细胞瘤中有 11例发生PTEN基因突变 ,突变率为 2 6 % (11/ 4 2 ) ,显著高于其它 6 0例胶质瘤 (χ2 =11.6 2 ,P <0 .0 1) ;胶质母细胞瘤Ki- 6 7的平均标记指数为 2 4 .6 1± 6 .81,其它胶质瘤的平均标记指数为 6 .72± 4 .6 8,后者Ki- 6 7指数显著小于前者 (u =5 .6 783,P <0 .0 1)。结论 :PTEN基因突变在胶质母细胞瘤的发生、发展中起重要作用 ;胶质瘤中Ki- 6 7的表达程度与病理恶性程度呈正相关 ;胶质母细胞瘤中PTEN基因突变与Ki- 6 7表达强度呈正相关。Objective:To research the PTEN gene(phosphatase and tensin homology deleted on chromosome ten gene) mutation and Ki-67 expression in glioblastomas, including their relations. Methods:The PTEN gene mutations were investigated by PCR-SSCP (single strand conformation polymorphism analysis of polymerase chain reaction products)from samples of 102 archival,formalin-fixed,paraffin-embedded gliomas samples;also the Ki-67 expressions were investigated by immunohistochemistry. Results:Eleven of PTEN gene mutations were identified in 42 glioblastomas, so the ratio of PTEN gene mutation in glioblastomas was 26%.It was significantly different from that of the other gliomas (χ 2=11.62, P <0.01 );the mean labeling index of Ki-67 positive expression in glioblastomas was 24.61±6.81,in other gliomas it was 6.72±4.68. The labeling index of Ki-67 positive expression in glioblastomas was significantly different from that of the other gliomas (u=5.6783, P <0.01). Conclusion: The PTEN gene mutation may have an important effect on the generation and development of glioblastoma;the degree of Ki-67 positive expression in glioma has positive correlation with gliomas malignancy;the PTEN gene mutation of glioblastoma has positive correlation with the degree of Ki-67 positive expression.

关 键 词：胶质母细胞瘤 PTEN基因 基因突变 KI-67 

分 类 号：R739.41[医药卫生—肿瘤]